Brand  (β version)

PDB ID: 6HG2

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Ligands
Code Name Style Show Link
FMT Formic acid PoSSuM
FOT 5-fluoro-2,6-dioxo-1,2,3,6-tetrahydropyrimidine-4-carboxylic acid PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
KCX Lysine nz-carboxylic acid
Glycosylation
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Structure summary

Code : 6HG2   PDBj   RCSB PDB   PDBe
Header : BIOSYNTHETIC PROTEIN
Title : Hybrid dihydroorotase domain of human CAD with E. coli flexible loop, bound to fluoroorotate
Release Data : 2018-10-24
Compound :
mol_id molecule chains
1 CAD protein A
ec: 6.3.5.5,2.1.3.2,3.5.2.3
mutation: Replacement of flexible loop: 1660-ANATTNSSHGVT-1569
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Homo sapiens  (taxid:9606)
gene: CAD
expression_system_cell_line: HEK293 GnTI
expression_system_plasmid: pOPIN-M
Authors : Ramon-Maiques, S., Del Cano-Ochoa, F.
Keywords : de novo pyrimidine biosynthesis, CAD, TIM-barrel, carboxylated lysine, BIOSYNTHETIC PROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.83 Å )
Citation :

Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD.

Del Cano-Ochoa, F.,Grande-Garcia, A.,Reverte-Lopez, M.  et al.
(2018)  J. Biol. Chem.  293 : 18903 - 18913

PubMed: 30315107
DOI: 10.1074/jbc.RA118.005494

Reaction

Chain : A
UniProt : P27708 (PYR1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate 6.3.5.5 PubMed:24332717
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H2O + L-glutamine = L-glutamate + NH4(+) 3.5.1.2 UniProtKB:P07259
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2 ATP + hydrogencarbonate + NH4(+) = 2 ADP + carbamoyl phosphate + 2 H(+) + phosphate 6.3.4.16 UniProtKB:P07259
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carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L- aspartate + phosphate 2.1.3.2 PubMed:24332717
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(S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate 3.5.2.3 PubMed:24332717
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