Brand  (β version)

PDB ID: 6HAE

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Ligands
Code Name Style Show Link
2PE Nonaethylene glycol PoSSuM
CL Chloride ion PoSSuM
E4M 1-{4-[(6s,6ar,7r)-3-amino-6,7-dimethyl-1-oxo-1,2,5,6,6a,7-hexahydro-8h-imidazo[1,5-f]pteridin-10-ium-8-yl]phenyl}-1-deoxy-5-O-{5-O-[(S)-{[(1s)-1,3-dicarboxypropyl]oxy}(hydroxy)phosphoryl]-alpha-D-ribofuranosyl}-D-ribitol PoSSuM
FE9 Iron-guanylyl pyridinol cofactor PoSSuM
GOL Glycerol PoSSuM
K Potassium ion PoSSuM
NA Sodium ion PoSSuM
SCN Thiocyanate ion PoSSuM
Non-standard Residues
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Structure summary

Code : 6HAE   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal structure of [Fe]-hydrogenase (Hmd) from Methanococcus aeolicus in complex with FeGP cofactor and methenyl-tetrahydromethanopterin (close form B)
Release Data : 2019-08-28
Compound :
mol_id molecule chains synonym
1 5,10-methenyltetrahydromethanopterin hydrogenase A,K H(2)-dependent methylene-H(4)MPT dehydrogenase,H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase,N(5),N(10)-methenyltetrahydromethanopterin hydrogenase
ec: 1.12.98.2
mutation: wild-type
Source :
mol_id organism_scientific expression_system
1 Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3)  (taxid:419665) Escherichia coli BL21(DE3)  (taxid:469008)
strain: ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3
variant: /
cell_line: /
atcc: /
organ: /
tissue: /
cell: /
gene: hmd, Maeo_1025
expression_system_variant: /
expression_system_cell_line: /
expression_system_atcc_number: /
expression_system_organ: /
expression_system_tissue: /
expression_system_cell: /
expression_system_vector_type: Plasmid
expression_system_plasmid: pET-24b+
Authors : Huang, G., Wagner, T., Wodrich, M.D., Ataka, K., Bill, E., Ermler, U., Hu, X., Shima, S.
Keywords : [Fe]-hydrogenase, catalytic cycle, conformational rearrangement, Fe-guanylylpyridinol cofactor, methanogenesis, hydride-transfer, tetrahydromethanopterin, C1-metabolism, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.85 Å )
Citation :

The atomic-resolution crystal structure of activated [Fe]-hydrogenase

Huang, G.,Wagner, T.,Wodrich, M.D.  et al.
(2019)  Nat Catal 

DOI: 10.1038/s41929-019-0289-4

Reaction

Chain : A, K
UniProt : A6UVT1 (A6UVT1_META3)
Reaction: EC: Evidence:
Physiological Direction:
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10- methylenetetrahydromethanopterin + H(+) 1.12.98.2 HAMAP-Rule:MF_01090
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