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Ligands
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Non-standard Residues
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FHE 2-[(4~{z})-2-[(1~{R})-1-azanyl-2-oxidanylidene-propyl]-4-[[4-[(2-nitrophenyl)methoxy]phenyl]methylidene]-5-oxidanylidene-imidazol-1-yl]ethanoic acid
Glycosylation
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Modification
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Code : 6H01   PDBj   RCSB PDB   PDBe
Header : FLUORESCENT PROTEIN
Title : Crystal structure of a domain-swapped dark-state sfGFP containing the unnatural amino acid ortho-nitrobenzyl-tyrosine (ONBY) at residue 66
Release Data : 2019-04-24
Compound :
mol_id molecule chains
1 Green fluorescent protein A,B,C,D
other_details: LIG contains the chromophore residues (threonine; ortho-nitrobenzyl-tyrosine; glycine)
Source :
mol_id organism_scientific organism_common expression_system
1 Aequorea victoria  (taxid:6100) Jellyfish Escherichia coli  (taxid:562)
gene: gfp
Authors : Kesgin-Schaefer, S., Tidow, H.
Keywords : GFP, unnatural amino acids, domain-swapped, FLUORESCENT PROTEIN
Exp. method : X-RAY DIFFRACTION ( 2.776 Å )
Citation :

Crystal structure of a domain-swapped photoactivatable sfGFP variant provides evidence for GFP folding pathway.

Kesgin-Schaefer, S.,Heidemann, J.,Puchert, A.  et al.
(2019)  Febs J.  286 : 2329 - 2340

PubMed: 30817081
DOI: 10.1111/febs.14797

Chain : A, B, C, D
UniProt : A0A059PIQ0 (A0A059PIQ0_AEQVI)
Reaction : -