Brand  (β version)

PDB ID: 6GXC

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Ligands
Code Name Style Show Link
FFK [(2~{R},3~{R},4~{R},5~{S},6~{R})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl] [oxidanyl-[(2~{z},6~{z},10~{z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl] hydrogen phosphate PoSSuM
MES 2-(N-morpholino)-ethanesulfonic acid PoSSuM
MN Manganese (II) ion PoSSuM
NA Sodium ion PoSSuM
PEG Di(hydroxyethyl)ether PoSSuM
Non-standard Residues
Code Name Show
DAB 2,4-diaminobutyric acid
PPN Para-nitrophenylalanine
Glycosylation
Code Name Emphasize
Modification
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Structure summary

Code : 6GXC   PDBj   RCSB PDB   PDBe
Header : MEMBRANE PROTEIN
Title : Bacterial oligosaccharyltransferase PglB in complex with an inhibitory peptide and a reactive lipid-linked oligosaccharide analog
Release Data : 2018-11-14
Compound :
mol_id molecule chains synonym
1 Undecaprenyl-diphosphooligosaccharide--protein glycotransferase A Protein glycosylation B
ec: 2.4.99.19
mol_id molecule chains
2 GLY-ASP-GLN-DAB-ALA-THR-PPN-GLY B
Source :
mol_id organism_scientific expression_system
1 Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060)  (taxid:306263) Escherichia coli BL21(DE3)  (taxid:469008)
strain: RM2100 / D67 / ATCC BAA-1060
gene: pglB, Cla_1253
mol_id organism_scientific expression_system
2 Campylobacter lari RM2100  (taxid:306263) Escherichia coli BL21(DE3)  (taxid:469008)
Authors : Napiorkowska, M., Locher, K.P., Boilevin, J., Darbre, T., Reymond, J.-L.
Keywords : Glycosyltransferase, Lipid-linked oligosaccharide, Ternary complex, MEMBRANE PROTEIN
Exp. method : X-RAY DIFFRACTION ( 3.401 Å )
Citation :

Structure of bacterial oligosaccharyltransferase PglB bound to a reactive LLO and an inhibitory peptide.

Napiorkowska, M.,Boilevin, J.,Darbre, T.  et al.
(2018)  Sci Rep  8 : 16297 - 16297

PubMed: 30389987
DOI: 10.1038/s41598-018-34534-0

Reaction

Chain : A
UniProt : B9KDD4 (PGLB_CAMLR)
Reaction: EC: Evidence:
Physiological Direction:
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine = tritrans,heptacis-undecaprenyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D- glycosyl linkage to protein L-asparagine. 2.4.99.19 PubMed:21677752, PubMed:24149797
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