Brand  (β version)

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
ADP Adenosine-5'-diphosphate
ATP Adenosine-5'-triphosphate
CL Chloride ion
EDO 1,2-ethanediol
FMN Flavin mononucleotide
GOL Glycerol
MG Magnesium ion
PO4 Phosphate ion
SO4 Sulfate ion
ZN Zinc ion
Modified Residues
Code Name Link
F6N 2-(aminomethyl)-1,3-oxazole-4-carboxylic acid
F75 2-(aminomethyl)-1,3-thiazole-4-carboxylic acid
OTZ 2-[2-(aminomethyl)-1,3-oxazol-4-yl]-1,3-thiazole-4-carboxylic acid
TOZ 2-[2-(aminomethyl)-1,3-thiazol-4-yl]-1,3-oxazole-4-carboxylic acid
Code : 6GRG
Header : BIOSYNTHETIC PROTEIN
Title : E. coli Microcin synthetase McbBCD complex with pro-MccB17, ADP and phosphate bound
Release Data : 2019-01-30
Compound :
mol_id molecule chains synonym
1 Bacteriocin microcin B17 A MccB17
other_details: The McbA protein was expressed with an eight-residue nickel affinity tag with sequence MGHHHHHH appended to the N-terminus of the full-length amino acid sequence. It was subsequently post-translationally modified by the McbBCD complex to yield nine heterocycles, where F6N equals oxazole and is derived from Gly-Ser, F75 equals thiazole and is derived from Gly-Cys, OTZ equals oxazole-thiazole and is derived from Gly-Ser-Cys, TOZ equals thiazole-oxazole and is derived from Gly-Cys-Ser. Each mono- or bis-heterocycle (where present) was treated as a pseudo-amino acid for refinement purposes. The numbering system used in this PDB file relates to the processed peptide where each pseudo-amino acid is treated as a single residue making the overall sequence nine residues shorter.
mol_id molecule chains
2 Microcin B17-processing protein McbB 1,2
mol_id molecule chains
3 Microcin B17-processing protein McbC C
mol_id molecule chains
4 Microcin B17-processing protein McbD D
other_details: There is a T171R substitution relative to UniProtKB sequence
Source :
mol_id organism_scientific expression_system
1 Escherichia coli str. K-12 substr. MG1655  (taxid:511145) Escherichia coli BW25113  (taxid:679895)
gene: mcbA
expression_system_plasmid: pBAD-McbABCDEFG
mol_id organism_scientific expression_system
2 Escherichia coli str. K-12 substr. MG1655  (taxid:511145) Escherichia coli BW25113  (taxid:679895)
gene: mcbB
expression_system_plasmid: pBAD-McbABCDEFG
mol_id organism_scientific expression_system
3 Escherichia coli str. K-12 substr. MG1655  (taxid:511145) Escherichia coli BW25113  (taxid:679895)
gene: mcbC
expression_system_plasmid: pBAD-McbABCDEFG
mol_id organism_scientific expression_system
4 Escherichia coli str. K-12 substr. MG1655  (taxid:511145) Escherichia coli BW25113  (taxid:679895)
gene: mcbD
expression_system_plasmid: pBAD-McbABCDEFG
Authors : Ghilarov, D., Stevenson, C.E.M., Travin, D.Y., Piskunova, J., Serebryakova, M., Maxwell, A., Lawson, D.M., Severinov, K.
Keywords : microcin, DNA gyrase, heterocyclization, posttranslational modification, BIOSYNTHETIC PROTEIN
Exp. method : X-RAY DIFFRACTION ( 2.3500 Å )
Citation :

Architecture of Microcin B17 Synthetase: An Octameric Protein Complex Converting a Ribosomally Synthesized Peptide into a DNA Gyrase Poison.

Ghilarov, D.,Stevenson, C.E.M.,Travin, D.Y.  et al.
(2019)  Mol. Cell  73 : 749 - 762.e5

PubMed: 30661981
DOI: 10.1016/j.molcel.2018.11.032

Chain : A
UniProt : P05834 (MCBA_ECOLX)
Reaction : -
Chain : 1, 2
UniProt : P23184 (MCBB_ECOLX)
Reaction : -
Chain : C
UniProt : P23185 (MCBC_ECOLX)
Reaction : -
Chain : D
UniProt : P23186 (MCBD_ECOLX)
Reaction : -