Brand  (β version)

PDB ID: 6GOS

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Ligands
Code Name Style Show Link
CL Chloride ion PoSSuM
EDO 1,2-ethanediol PoSSuM
FMN Flavin mononucleotide PoSSuM
GOL Glycerol PoSSuM
SO4 Sulfate ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
F6N 2-(aminomethyl)-1,3-oxazole-4-carboxylic acid
F75 2-(aminomethyl)-1,3-thiazole-4-carboxylic acid
OTZ 2-[2-(aminomethyl)-1,3-oxazol-4-yl]-1,3-thiazole-4-carboxylic acid
TOZ 2-[2-(aminomethyl)-1,3-thiazol-4-yl]-1,3-oxazole-4-carboxylic acid
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 6GOS   PDBj   RCSB PDB   PDBe
Header : Antibiotic/inhibitor
Title : E. coli Microcin synthetase McbBCD complex with pro-MccB17 bound
Release Data : 2019-01-30
Compound :
mol_id molecule chains synonym
1 Bacteriocin microcin B17 A MccB17
other_details: The McbA protein was expressed with an eight-residue nickel affinity tag with sequence MGHHHHHH appended to the N-terminus of the full-length amino acid sequence. It was subsequently post-translationally modified by the McbBCD complex to yield nine heterocycles, where F6N equals oxazole and is derived from Gly-Ser, F75 equals thiazole and is derived from Gly-Cys, OTZ equals oxazole-thiazole and is derived from Gly-Ser-Cys, TOZ equals thiazole-oxazole and is derived from Gly-Cys-Ser. Each mono- or bis-heterocycle (where present) was treated as a pseudo-amino acid for refinement purposes. The numbering system used in the model file relates to the processed peptide where each pseudo-amino acid is treated as a single residue making the overall sequence nine residues shorter.
mol_id molecule chains
2 Microcin B17-processing protein McbB 1,2
mol_id molecule chains
3 Microcin B17-processing protein McbC C
mol_id molecule chains
4 Microcin B17-processing protein McbD D
Source :
mol_id organism_scientific expression_system
1 Escherichia coli str. K-12 substr. MG1655  (taxid:511145) Escherichia coli BW25113  (taxid:679895)
gene: mcbA
expression_system_plasmid: pBAD-HisMcbABCDEFG
mol_id organism_scientific expression_system
2 Escherichia coli str. K-12 substr. MG1655  (taxid:511145) Escherichia coli BW25113  (taxid:679895)
gene: mcbB
expression_system_plasmid: pBAD-HisMcbABCDEFG
mol_id organism_scientific expression_system
3 Escherichia coli str. K-12 substr. MG1655  (taxid:511145) Escherichia coli BW25113  (taxid:679895)
gene: mcbC
expression_system_plasmid: pBAD-McbABCDEFG
mol_id organism_scientific expression_system
4 Escherichia coli str. K-12 substr. MG1655  (taxid:511145) Escherichia coli BW25113  (taxid:679895)
gene: mcbD
expression_system_plasmid: pBAD-McbABCDEFG
Authors : Ghilarov, D., Stevenson, C.E.M., Travin, D.Y., Piskunova, J., Serebryakova, M., Maxwell, A., Lawson, D.M., Severinov, K.
Keywords : microcin, DNA gyrase, heterocyclization, posttranslational modification, BIOSYNTHETIC PROTEIN, Antibiotic-inhibitor complex
Exp. method : X-RAY DIFFRACTION ( 2.10 Å )
Citation :

Architecture of Microcin B17 Synthetase: An Octameric Protein Complex Converting a Ribosomally Synthesized Peptide into a DNA Gyrase Poison.

Ghilarov, D.,Stevenson, C.E.M.,Travin, D.Y.  et al.
(2019)  Mol. Cell  73 : 749 - 762.e5

PubMed: 30661981
DOI: 10.1016/j.molcel.2018.11.032

Reaction

Chain : A
UniProt : P05834 (MCBA_ECOLX)
Reaction : -
Chain : 1, 2
UniProt : P23184 (MCBB_ECOLX)
Reaction : -
Chain : C
UniProt : P23185 (MCBC_ECOLX)
Reaction : -
Chain : D
UniProt : P23186 (MCBD_ECOLX)
Reaction : -