Het-PDB Navi2 (PDB: 6GCF)

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Ligands
Code	Name		Style	Show	Link

Non-standard Residues
Code	Name	Show
C34	N4-methyl-2'-deoxy-cytidine-5'-monophosphate

Glycosylation
Code	Name	Emphasize

Modification
Code	Name	Show

Download interaction data: 6GCF

Code :

6GCF PDBj RCSB PDB PDBe

Header :

DNA BINDING PROTEIN

Title :

DNA binding domain of restriction endonuclease McrBC in complex with N4-methylcytosine DNA

Release Data :

2018-09-19

Compound :

mol_id	molecule	chains	synonym
1	5-methylcytosine-specific restriction enzyme B	A,B	EcoKMcrBC
	ec: 3.1.21.-

mol_id	molecule	chains
2	DNA (5'-D(GPAPGPAPCPCPGPGPTPAPGPC)-3')	C

mol_id	molecule	chains
3	DNA (5'-D(GPCPTPAP(C34)PCPGPGPTPCPTPC)-3')	D

Source :

mol_id	organism_scientific	expression_system
1	Escherichia coli (taxid:83333)	Escherichia coli str. K-12 substr. DH10B (taxid:316385)
	strain: K12 gene: mcrB, rglB, b4346, JW5871

mol_id	organism_scientific
2	Synthetic construct (taxid:32630)
	synthetic: yes

mol_id	organism_scientific
3	Synthetic construct (taxid:32630)
	synthetic: yes

Authors :

Sasnauskas, G.

Keywords :

N4-methylcytosine, restriction endonuclease, McrBC, base flipping, DNA BINDING PROTEIN

Exp. method :

X-RAY DIFFRACTION ( 1.55 Å )

Citation :

Recognition of modified cytosine variants by the DNA-binding domain of methyl-directed endonuclease McrBC.

Zagorskaite, E.,Manakova, E.,Sasnauskas, G.
(2018) FEBS Lett. 592 : 3335 - 3345

PDB ID: 6GCF