Brand  (β version)

PDB ID: 6FH8

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Ligands
Code Name Style Show Link
JCT Biotinylated ruthenium cyclopentadienide PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 6FH8   PDBj   RCSB PDB   PDBe
Header : biotin binding protein
Title : E. coli surface display of streptavidin for directed evolution of an allylic deallocase
Release Data : 2018-08-22
Compound :
mol_id molecule chains
1 Streptavidin A
mutation: S112M-K121A
Source :
mol_id organism_scientific expression_system
1 Streptomyces avidinii  (taxid:1895) Escherichia coli  (taxid:562)
Authors : Heinisch, T., Schwizer, F., Garabedian, B., Csibra, E., Jeschek, M., Pinheiro Bernhardes, V., Marliere, P., Panke, S., Ward, T.R.
Keywords : biotin binding protein, artificial metalloenzyme, E. coli surface display, streptavidin, biotin, bioorthogonal reaction, uncaging, allyl deprotection, directed evolution
Exp. method : X-RAY DIFFRACTION ( 1.64 Å )
Citation :

E. colisurface display of streptavidin for directed evolution of an allylic deallylase.

Heinisch, T.,Schwizer, F.,Garabedian, B.  et al.
(2018)  Chem Sci  9 : 5383 - 5388

PubMed: 30079176
DOI: 10.1039/c8sc00484f

Reaction

Chain : A
UniProt : P22629 (SAV_STRAV)
Reaction : -