Brand  (β version)

PDB ID: 6FFB

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Ligands
Code Name Style Show Link
F45 [6-(3,4-dihydroxyphenyl)pyridin-2-yl](4-fluoro-3-hydroxyphenyl)methanone PoSSuM
NA Sodium ion PoSSuM
NAD Nicotinamide-adenine-dinucleotide PoSSuM
BGC Beta-D-glucopyranose PoSSuM
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Structure summary

Code : 6FFB   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : 17beta-hydroxysteroid dehydrogenase 14 variant S205 - mutant Q148A - in complex with a nonsteroidal inhibitor
Release Data : 2019-01-30
Compound :
mol_id molecule chains synonym
1 17-beta-hydroxysteroid dehydrogenase 14 A 17-beta-HSD 14,17-beta-hydroxysteroid dehydrogenase DHRS10,Dehydrogenase/reductase SDR family member 10,Retinal short-chain dehydrogenase/reductase retSDR3,Short chain dehydrogenase/reductase family 47C member 1
ec: 1.1.1.-
mutation: Q148A
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli BL21(DE3)  (taxid:469008)
gene: HSD17B14, DHRS10, SDR3, SDR47C1, UNQ502/PRO474
expression_system_variant: pLysS
Authors : Bertoletti, N., Heine, A., Klebe, G., Marchais-Oberwinkler, S.
Keywords : 17b-HSD14, mutation, inhibition, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.65 Å )
Citation :

Mutational and structural studies uncover crucial amino acids determining activity and stability of 17 beta-HSD14.

Badran, M.J.,Bertoletti, N.,Keils, A.  et al.
(2019)  J.Steroid Biochem.Mol.Biol.  189 : 135 - 144

PubMed: 30836176
DOI: 10.1016/j.jsbmb.2019.02.009

Reaction

Chain : A
UniProt : Q9BPX1 (DHB14_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
17beta-estradiol + NAD(+) = estrone + H(+) + NADH 1.1.1.62 PubMed:17067289
left-to-right
androst-5-en-3beta,17beta-diol + NAD(+) = 3beta- hydroxyandrost-5-en-17-one + H(+) + NADH - PubMed:17067289
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