Brand  (β version)

PDB ID: 6F2N

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Code Name Style Show Link
CF8 (~{z})-3-[2-(naphthalen-2-ylmethyl)phenyl]-2-sulfanyl-prop-2-enoic acid PoSSuM
SO4 Sulfate ion PoSSuM
ZN Zinc ion PoSSuM

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Structure summary

Code : 6F2N   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal structure of BCII Metallo-beta-lactamase in complex with KDU197
Release Data : 2018-10-03
Compound :
mol_id molecule chains synonym
1 Metallo-beta-lactamase type 2 A B2 metallo-beta-lactamase,Beta-lactamase II,Cephalosporinase,Metallo-beta-lactamase type II,Metallothioprotein beta-lactamase II,Penicillinase,Zinc-requiring beta-lactamase II
ec: 3.5.2.6
Source :
mol_id organism_scientific expression_system
1 Bacillus cereus  (taxid:1396) Escherichia coli 'BL21-Gold(DE3)pLysS AG'  (taxid:866768)
gene: blm
expression_system_vector_type: plasmid
expression_system_plasmid: PET9A
Authors : McDonough, M.A., El-Hussein, A., Schofield, C.J., Zhang, D., Brem, J.
Keywords : ANTIMICROBIAL RESISTANCE, METALLO BETA LACTAMASE, INHIBITOR, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.1490 Å )
Citation :

Structure activity relationship studies on rhodanines and derived enethiol inhibitors of metallo-beta-lactamases.

Zhang, D.,Markoulides, M.S.,Stepanovs, D.  et al.
(2018)  Bioorg. Med. Chem.  26 : 2928 - 2936

PubMed: 29655609
DOI: 10.1016/j.bmc.2018.02.043

Reaction

Chain : A
UniProt : P04190 (BLA2_BACCE)
Reaction: EC: Evidence:
Physiological Direction:
a beta-lactam + H2O = a substituted beta-amino acid 3.5.2.6 UniProtKB:P25910
-
Cofactor: Evidence: Note:
Zn(2+) ECO:0000269 | PubMed:20677753
ECO:0000269 | PubMed:24059435
ECO:0000269 | PubMed:26482303
ECO:0000269 | PubMed:7588620
ECO:0000269 | PubMed:9761898
Binds 2 Zn(2+) ions per subunit. The enzyme can also function with only 1 Zn(2+) ion (PubMed:9761898).