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Ligands
Code Name Style Show Link
ZN Zinc ion
CNN Cyanamide
SO4 Sulfate ion
Non-standard Residues
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Glycosylation
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Code : 6DKA   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : Yeast Ddi2 Cyanamide Hydratase
Release Data : 2019-05-08
Compound :
mol_id molecule chains
1 DNA damage-inducible protein A,B,C,D,E,F,G,H,I
mutation: T157V
Source :
mol_id organism_scientific organism_common expression_system
1 Saccharomyces cerevisiae (strain YJM789)  (taxid:307796) Baker's yeast Escherichia coli BL21(DE3)  (taxid:469008)
strain: YJM789
gene: SCY_1694
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: PGEX-6P1
Authors : Moore, S.A., Xiao, W., Li, J.
Keywords : Zn-metalloprotein, HD-domain, hydratase, cyanamide, METAL BINDING PROTEIN, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.901 Å )
Citation :

Structure of Ddi2, a highly inducible detoxifying metalloenzyme fromSaccharomyces cerevisiae.

Li, J.,Jia, Y.,Lin, A.  et al.
(2019)  J.Biol.Chem.  294 : 10674 - 10685

PubMed: 31152065
DOI: 10.1074/jbc.RA118.006394

Chain : A, B, C, D, E, F, G, H, I
UniProt : A7A1Y4 (A7A1Y4_YEAS7)
Reaction: EC: Evidence:
Physiological Direction:
urea = cyanamide + H2O 4.2.1.69 ARBA:ARBA00036058
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Cofactor: Evidence: Note:
Zn(2+) ECO:0000256 | ARBA:ARBA00001947
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