Brand  (β version)

PDB ID: 6DE9

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Ligands
Code Name Style Show Link
FES Fe2/s2 (inorganic) cluster PoSSuM
FUN 5-(aminosulfonyl)-4-chloro-2-[(2-furylmethyl)amino]benzoic acid PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 6DE9   PDBj   RCSB PDB   PDBe
Header : METAL BINDING PROTEIN
Title : mitoNEET bound to furosemide
Release Data : 2019-05-15
Compound :
mol_id molecule chains synonym
1 CDGSH iron-sulfur domain-containing protein 1 A MitoNEET
fragment: UNP residues 32-108
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli BL21(DE3)  (taxid:469008)
gene: CISD1, C10orf70, ZCD1, MDS029
expression_system_vector_type: plasmid
expression_system_plasmid: pET11a-His-SUMO-mitoNEET
Authors : Robart, A.R., Geldenhuys, W.J.
Keywords : mitoNEET, FES, furosemide, METAL BINDING PROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.95 Å )
Citation :

Crystal structure of the mitochondrial protein mitoNEET bound to a benze-sulfonide ligand.

Geldenhuys, W.J.,Long, T.E.,Saralkar, P.  et al.
(2019)  Commun Chem  2

PubMed: 32382661
DOI: 10.1038/s42004-019-0172-x

Reaction

Chain : A
UniProt : Q9NZ45 (CISD1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
L-cysteine + 2-oxoglutarate = 2-oxo-3-sulfanylpropanoate + L- glutamate 2.6.1.3 PubMed:36194135
right-to-left
Cofactor: Evidence: Note:
[2Fe-2S] cluster ECO:0000269 | PubMed:17376863
ECO:0000269 | PubMed:17584744
ECO:0000269 | PubMed:17766439
ECO:0000269 | PubMed:17766440
ECO:0000269 | PubMed:17905743
ECO:0000269 | PubMed:23758282
Binds 1 [2Fe-2S] cluster per subunit. The [2Fe-2S] cluster is redox-active and pH labile and is significantly less stable at pH 4.5 as compared with pH 7.0.
pyridoxal 5'-phosphate ECO:0000269 | PubMed:36194135
Binds 1 [2Fe-2S] cluster per subunit. The [2Fe-2S] cluster is redox-active and pH labile and is significantly less stable at pH 4.5 as compared with pH 7.0.