Brand  (β version)

PDB ID: 6CUT

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Ligands
Code Name Style Show Link
FEJ (2s,3s)-3-hydroxy-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]-4-methylpentanoic acid (non-preferred name) PoSSuM
NA Sodium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 6CUT   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : Engineered Holo TrpB from Pyrococcus furiosus, PfTrpB7E6 with (2S,3S)-isopropylserine bound as the external aldimine
Release Data : 2018-09-26
Compound :
mol_id molecule chains
1 Tryptophan synthase beta chain 1 A,B,C,D
ec: 4.2.1.20
mutation: I16V, E17G, L91P, F95L, L161A, V173E, F274L, T292S, V384A
Source :
mol_id organism_scientific expression_system
1 Pyrococcus furiosus  (taxid:186497) Escherichia coli  (taxid:469008)
strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1
gene: trpB1, PF1706
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET22
Authors : Boville, C.E., Scheele, R.A., Buller, A.R., Arnold, F.H.
Keywords : external aldimine, (2S, 3S)-isopropylserine, LYASE
Exp. method : X-RAY DIFFRACTION ( 1.7700 Å )
Citation :

Engineered Biosynthesis of beta-Alkyl Tryptophan Analogues.

Boville, C.E.,Scheele, R.A.,Koch, P.  et al.
(2018)  Angew. Chem. Int. Ed. Engl.  57 : 14764 - 14768

PubMed: 30215880
DOI: 10.1002/anie.201807998

Reaction

Chain : A, B, C, D
UniProt : Q8U093 (TRPB1_PYRFU)
Reaction: EC: Evidence:
Physiological Direction:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D- glyceraldehyde 3-phosphate + L-tryptophan + H2O 4.2.1.20 -
-
Cofactor: Evidence: Note:
pyridoxal 5'-phosphate ECO:0000250
-