Brand  (β version)

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
CL Chloride ion
FDJ 5'-O-[(R)-{[(R)-[(R)-chloro(phosphono)methyl](hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]thymidine
MG Magnesium ion
NA Sodium ion
Modified Residues
Code Name Link
DOC 2',3'-dideoxycytidine-5'-monophosphate
Code : 6CTM
Header : transcription/dna
Title : Ternary complex crystal structure of DNA polymerase Beta with a dideoxy terminated primer with CHCL(R-isomer), beta, gamma dTTP analogue
Release Data : 2018-06-20
Compound :
mol_id molecule chains
1 DNA (5'-D(*CP*CP*GP*AP*CP*AP*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3') T
mol_id molecule chains
2 DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*(DOC))-3') P
mol_id molecule chains
3 DNA (5'-D(P*GP*TP*CP*GP*G)-3') D
mol_id molecule chains
4 DNA polymerase beta A
ec: 2.7.7.7,4.2.99.-
Source :
mol_id organism_scientific
1 Homo sapiens  (taxid:9606)
synthetic: yes
mol_id organism_scientific
2 Homo sapiens  (taxid:9606)
synthetic: yes
mol_id organism_scientific
3 Homo sapiens  (taxid:9606)
synthetic: yes
mol_id organism_scientific organism_common expression_system
4 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: POLB
expression_system_strain: Tap56
expression_system_vector_type: Plasmid
expression_system_plasmid: pwl-11
Authors : Batra, V.K., Wilson, S.H.
Keywords : DNA Polymerase Beta, Conformational Change, enzyme mechanism, LFER, transcription-dna complex
Exp. method : X-RAY DIFFRACTION ( 2.101 Å )
Citation :

Mapping Functional Substrate-Enzyme Interactions in the pol beta Active Site through Chemical Biology: Structural Responses to Acidity Modification of Incoming dNTPs.

Batra, V.K.,Oertell, K.,Beard, W.A.  et al.
(2018)  Biochemistry  57 : 3934 - 3944

PubMed: 29874056
DOI: 10.1021/acs.biochem.8b00418

Chain : A
UniProt : P06746 (DPOLB_HUMAN)
Reaction : Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).