Brand  (β version)

PDB ID: 6B1S

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Ligands
Code Name Style Show Link
Non-standard Residues
Code Name Show
CGY (1r)-1,4-anhydro-2-deoxy-1-[2,6-diamino-5-(dihydroxyamino)pyridin-3-yl]-5-O-phosphono-D-erythro-pentitol
Glycosylation
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Modification
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Structure summary

Code : 6B1S   PDBj   RCSB PDB   PDBe
Header : DNA BINDING PROTEIN/DNA
Title : Hydrogen Bonding Complementary, not size complementarity is key in the formation of the double helix
Release Data : 2018-09-19
Compound :
mol_id molecule chains
1 Reverse transcriptase A,B
ec: 2.7.7.49
fragment: Catalytic fragment (UNP residues 683-937)
mol_id molecule chains
2 DNA (5'-D(*CP*TP*TP*AP*TP*AP*(CGY)P*(CGY)P*TP*TP*TP*AP*TP*AP*AP*G)-3') E,F
Source :
mol_id organism_scientific organism_common expression_system
1 Moloney murine leukemia virus  (taxid:11801) MoMLV Escherichia coli  (taxid:562)
mol_id organism_scientific
2 Escherichia coli  (taxid:562)
synthetic: yes
Authors : Singh, I., Georgiadis, M.M.
Keywords : Protein-DNA, AEGIS, unnatural base pair, host-guest system, DNA BINDING PROTEIN, DNA BINDING PROTEIN-DNA complex
Exp. method : X-RAY DIFFRACTION ( 2.000 Å )
Citation :

"Skinny" and "Fat" DNA: Two New Double Helices.

Hoshika, S.,Singh, I.,Switzer, C.  et al.
(2018)  J. Am. Chem. Soc.  140 : 11655 - 11660

PubMed: 30148365
DOI: 10.1021/jacs.8b05042

Reaction

Chain : A, B
UniProt : P03355 (POL_MLVMS)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.49 PROSITE-ProRule:PRU00405
-
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 PROSITE-ProRule:PRU00405
-
Endonucleolytic cleavage to 5'-phosphomonoester. 3.1.26.4 PROSITE-ProRule:PRU00408
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000255 | PROSITE-ProRule:PRU00405
The RT polymerase active site binds 2 magnesium ions.
Mg(2+) ECO:0000250 | UniProtKB:Q2F7J3
ECO:0000269 | PubMed:16912289
Binds 2 Mn(2+)/Mg(2+) ions for ribonuclease H (RNase H) activity.
Mg(2+) ECO:0000305
Magnesium ions are required for integrase activity. Binds at least 1, maybe 2 magnesium ions.