Brand  (β version)

PDB ID: 6ASN

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Ligands
Code Name Style Show Link
03S Methanesulfonic acid PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 6ASN   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : E. coli phosphoenolpyruvate carboxykinase K212I F216V mutant bound to methanesulfonate
Release Data : 2018-08-29
Compound :
mol_id molecule chains synonym
1 Phosphoenolpyruvate carboxykinase (ATP) A PEPCK
ec: 4.1.1.49
mutation: K212I, F216V
Source :
mol_id organism_scientific expression_system
1 Escherichia coli (strain K12)  (taxid:83333) Escherichia coli  (taxid:562)
strain: K12
gene: pckA, pck, b3403, JW3366
Authors : Tang, H.Y.H., Shin, D.S., Tainer, J.A.
Keywords : Nonnative ligand, LYASE
Exp. method : X-RAY DIFFRACTION ( 1.549 Å )
Citation :

Structural Control of Nonnative Ligand Binding in Engineered Mutants of Phosphoenolpyruvate Carboxykinase.

Tang, H.Y.H.,Shin, D.S.,Hura, G.L.  et al.
(2018)  Biochemistry  57 : 6688 - 6700

PubMed: 30376300
DOI: 10.1021/acs.biochem.8b00963

Reaction

Chain : A
UniProt : P22259 (PCKA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
oxaloacetate + ATP = phosphoenolpyruvate + ADP + CO2 4.1.1.49 HAMAP-Rule:MF_00453, PubMed:6986370
-
Cofactor: Evidence: Note:
Mn(2+) ECO:0000255 | HAMAP-Rule:MF_00453
ECO:0000269 | PubMed:1701430
ECO:0000269 | PubMed:17475535
ECO:0000269 | PubMed:9406547
ECO:0000269 | Ref.20
ECO:0000269 | Ref.21
Binds 1 Mn(2+) ion per subunit.