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C06 [[(2s,3r,4s,5r)-5-(2-azanyl-6-oxidanylidene-1h-purin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2s,3s,4s,5r,6r)-6-methyl-3,4,5-tris(oxidanyl)oxan-2-yl] hydrogen phosphate
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Glycosylation
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Code : 5ZOI   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal Structure of alpha1,3-Fucosyltransferase
Release Data : 2019-06-26
Compound :
mol_id molecule chains synonym
1 Alpha-(1,3)-fucosyltransferase FucT A,B 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase
ec: 2.4.1.152
fragment: UNP residues 1-412
mutation: S45F, D127N, R128E, H131I, Y199N, D340D, V368A
Source :
mol_id organism_scientific organism_common expression_system
1 Helicobacter pylori  (taxid:210) Campylobacter pylori Escherichia coli  (taxid:562)
gene: fucT
Authors : Tan, Y., Yang, G.
Keywords : enzyme, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 3.19 Å )
Citation :

Directed evolution of an alpha 1,3-fucosyltransferase using a single-cell ultrahigh-throughput screening method.

Tan, Y.,Zhang, Y.,Han, Y.  et al.
(2019)  Sci Adv  5 : eaaw8451 - eaaw8451

PubMed: 31633018
DOI: 10.1126/sciadv.aaw8451

Chain : A, B
UniProt : O30511 (FUCT_HELPX)
Reaction: EC: Evidence:
Physiological Direction:
a beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + GDP-beta-L-fucose = a beta-D-galactosyl-(1->4)-[alpha-L- fucosyl-(1->3)]-N-acetyl-beta-D-glucosaminyl derivative + GDP + H(+) 2.4.1.152 PubMed:12676935, PubMed:16150700, PubMed:16800635, PubMed:17251184, PubMed:9261149
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