Brand  (β version)

PDB ID: 5Y8R

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Ligands
Code Name Style Show Link
Non-standard Residues
Code Name Show
CH7 [(4z)-4-(4-hydroxybenzylidene)-5-oxo-2-(3,4,5,6-tetrahydropyridin-2-yl)-4,5-dihydro-1h-imidazol-1-yl]acetic acid
NFA Phenylalanine amide
Glycosylation
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Structure summary

Code : 5Y8R   PDBj   RCSB PDB   PDBe
Header : FLUORESCENT PROTEIN
Title : ZsYellow at pH 3.5
Release Data : 2017-09-13
Compound :
mol_id molecule chains synonym
1 GFP-like fluorescent chromoprotein FP538 A zFP538
mutation: M129V
Source :
mol_id organism_scientific organism_common expression_system
1 Zoanthus sp.  (taxid:105402) Green polyp Escherichia coli BL21(DE3)  (taxid:469008)
expression_system_strain: BL21(DE3)
Authors : Bae, J.E., Kim, I.J., Nam, K.H.
Keywords : ZsYellow, pH, FLUORESCENT PROTEIN
Exp. method : X-RAY DIFFRACTION ( 2.3000 Å )
Citation :

Disruption of the hydrogen bonding network determines the pH-induced non-fluorescent state of the fluorescent protein ZsYellow by protonation of Glu221.

Bae, J.E.,Kim, I.J.,Nam, K.H.
(2017)  Biochem. Biophys. Res. Commun.  493 : 562 - 567

PubMed: 28867188
DOI: 10.1016/j.bbrc.2017.08.152

Reaction

Chain : A
UniProt : Q9U6Y4 (GFPL2_ZOASP)
Reaction : -