Brand  (β version)

PDB ID: 5JS2

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Ligands
Code Name Style Show Link
IPH Phenol PoSSuM
MG Magnesium ion PoSSuM
PO4 Phosphate ion PoSSuM
Non-standard Residues
Code Name Show
6NW 2'-O-methyl-5'-O-thiophosphonoadenosine
6OO 2'-O-methyl-5'-O-thiophosphonocytidine
6OP 1-[(5e)-5,6-dideoxy-2-O-(2-methoxyethyl)-6-phosphono-beta-D-ribo-hex-5-enofuranosyl]-5-methylpyrimidine-2,4(1h,3h)-dione
F2T 2'-deoxy-2'-fluoro-5'-O-thiophosphonouridine
UFT 2'-deoxy-2'-fluorouridine 5'-(dihydrogen phosphate)
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 5JS2   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/RNA
Title : Human Argonaute-2 Bound to a Modified siRNA
Release Data : 2016-07-20
Compound :
mol_id molecule chains synonym
1 Protein argonaute-2 A hAgo2,Argonaute RISC catalytic component 2,Eukaryotic translation initiation factor 2C 2,eIF2C 2,PAZ Piwi domain protein,PPD,Protein slicer
ec: 3.1.26.-
mutation: S387D
mol_id molecule chains
2 modified siRNA B
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Spodoptera frugiperda  (taxid:7108)
gene: AGO2, EIF2C2
mol_id organism_scientific
2 Synthetic construct  (taxid:32630)
synthetic: yes
Authors : Schirle, N.T., MacRae, I.J.
Keywords : Argonaute, siRNA, RNAi, nuclease, HYDROLASE-RNA complex
Exp. method : X-RAY DIFFRACTION ( 2.954 Å )
Citation :

Structural Analysis of Human Argonaute-2 Bound to a Modified siRNA Guide.

Schirle, N.T.,Kinberger, G.A.,Murray, H.F.  et al.
(2016)  J.Am.Chem.Soc.  138 : 8694 - 8697

PubMed: 27380263
DOI: 10.1021/jacs.6b04454

Reaction

Chain : A
UniProt : Q9UKV8 (AGO2_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
Endonucleolytic cleavage to 5'-phosphomonoester. 3.1.26.n2 HAMAP-Rule:MF_03031, PubMed:15105377, PubMed:23746446
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Cofactor: Evidence: Note:
Mn(2+) ECO:0000269 | PubMed:15284456
ECO:0000269 | PubMed:15284456
-