Brand  (β version)

PDB ID: 5IED

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Color scheme of protein:

Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
CTS Castanospermine PoSSuM
EDO 1,2-ethanediol PoSSuM
FMT Formic acid PoSSuM
P6G Hexaethylene glycol PoSSuM
PG4 Tetraethylene glycol PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
NAG 2-acetamido-2-deoxy-beta-D-glucopyranose
Modification
Code Name Show

Structure summary

Code : 5IED   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Murine endoplasmic reticulum alpha-glucosidase II with castanospermine
Release Data : 2016-07-27
Compound :
mol_id molecule chains synonym
1 Neutral alpha-glucosidase AB A Alpha-glucosidase 2,Glucosidase II subunit alpha
ec: 3.2.1.84
other_details: This chain contains all of the residues from the mature Q8BHN3 isoform 2 (i.e. no signal peptide and starts at residue 33) but has been trypsinised so that there are two gaps in the sequence between: 186-243 and 351-369 (inclusive)
mol_id molecule chains synonym
2 Glucosidase 2 subunit beta B 80K-H protein,Glucosidase II subunit beta,Protein kinase C substrate 60.1 kDa protein heavy chain,PKCSH
other_details: This chain contains all of the residues from the mature O08795 but has been trypsinised so that there all that was crystallised is: 30-117
Source :
mol_id organism_scientific organism_common expression_system
1 Mus musculus  (taxid:10090) Mouse Homo sapiens  (taxid:9606)
gene: Ganab, G2an, Kiaa0088
expression_system_common: Human
expression_system_cell_line: HEK 293-F
expression_system_cell: ENDOTHELIAL
expression_system_vector_type: PLASMID
expression_system_plasmid: pOPINGS
mol_id organism_scientific organism_common expression_system
2 Mus musculus  (taxid:10090) Mouse Homo sapiens  (taxid:9606)
gene: Prkcsh
expression_system_common: Human
expression_system_cell_line: HEK 293-F
expression_system_organ: KIDNEY
expression_system_cell: ENDOTHELIAL
expression_system_vector_type: PLASMID
expression_system_plasmid: pOPING
Authors : Caputo, A.T., Roversi, P., Alonzi, D.S., Kiappes, J.L., Zitzmann, N.
Keywords : Enzyme Glycosyl hydrolase GH31 Quality control exoglycosidase, hydrolase
Exp. method : X-RAY DIFFRACTION ( 1.81 Å )
Citation :

Structures of mammalian ER alpha-glucosidase II capture the binding modes of broad-spectrum iminosugar antivirals.

Caputo, A.T.,Alonzi, D.S.,Marti, L.  et al.
(2016)  Proc.Natl.Acad.Sci.USA  113 : E4630 - E4638

PubMed: 27462106
DOI: 10.1073/pnas.1604463113

Reaction

Chain : A
UniProt : Q8BHN3 (GANAB_MOUSE)
Reaction: EC: Evidence:
Physiological Direction:
N(4)-(alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man- (1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)- [alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D- Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl- [protein] + H2O = N(4)-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha- D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man- (1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)- beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + beta-D-glucose 3.2.1.207 PubMed:27462106
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N(4)-(alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man- (1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)- alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D- Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L- asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1->3)-alpha-D-Man- (1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)- alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D- Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L- asparaginyl-[protein] + beta-D-glucose 3.2.1.207 PubMed:27462106
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Chain : B
UniProt : O08795 (GLU2B_MOUSE)
Reaction : -