Brand  (β version)

PDB ID: 5EY8

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Ligands
Code Name Style Show Link
5SV [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl icosyl hydrogen phosphate PoSSuM
Non-standard Residues
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Structure summary

Code : 5EY8   PDBj   RCSB PDB   PDBe
Header : LIGASE
Title : Structure of FadD32 from Mycobacterium smegmatis complexed to AMPC20
Release Data : 2015-12-16
Compound :
mol_id molecule chains synonym
1 Acyl-CoA synthase A,B,C,D,E,F,G,H Fatty-acid-CoA ligase FadD32
Source :
mol_id organism_scientific expression_system
1 Mycobacterium smegmatis  (taxid:1772) Escherichia coli BL21(DE3)  (taxid:469008)
gene: fadD32, MSMEG_6393, MSMEI_6225
expression_system_variant: Star One Shot
expression_system_vector_type: plasmid
expression_system_vector: pET15b
Authors : Guillet, V., Maveyraud, L., Mourey, L.
Keywords : Ligase, fatty-acyl AMP ligase
Exp. method : X-RAY DIFFRACTION ( 3.500 Å )
Citation :

Insight into Structure-Function Relationships and Inhibition of the Fatty Acyl-AMP Ligase (FadD32) Orthologs from Mycobacteria.

Guillet, V.,Galandrin, S.,Maveyraud, L.  et al.
(2016)  J.Biol.Chem.  291 : 7973 - 7989

PubMed: 26900152
DOI: 10.1074/jbc.M115.712612

Reaction

Chain : A, B, C, D, E, F, G, H
UniProt : A0R618 (FAA32_MYCS2)
Reaction: EC: Evidence:
Physiological Direction:
a long-chain fatty acid + ATP + holo-[ACP] = a long-chain fatty acyl-[ACP] + AMP + diphosphate 6.2.1.20 UniProtKB:O53580
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ATP + decanoate + H(+) = decanoyl-AMP + diphosphate - PubMed:23364516
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ATP + dodecanoate + H(+) = diphosphate + dodecanoyl-AMP - PubMed:23364516, PubMed:26900152
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ATP + H(+) + tetradecanoate = diphosphate + tetradecanoyl-AMP - PubMed:23364516, PubMed:26900152
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