Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
FFF Trifluorofurnesyl diphosphate
MG Magnesium ion
Code : 5EAU
Header : ISOPRENOID SYNTHASE
Title : 5-EPI-ARISTOLOCHENE SYNTHASE FROM NICOTIANA TABACUM
Release Data : 1998-04-08
Compound :
mol_id molecule chains synonym
1 5-EPI-ARISTOLOCHENE SYNTHASE A 5-EPI-ARISTOLOCHENE CYCLASE
mutation: EXPRESSED WITH 6-HIS TAG
Source :
mol_id organism_scientific organism_common expression_system
1 Nicotiana tabacum  (taxid:4097) Common tobacco Escherichia coli BL21(DE3)  (taxid:469008)
strain: BL21 (DE3)
cell_line: BL21
cellular_location: CYTOPLASM
expression_system_strain: BL21 (DE3)
expression_system_cellular_location: CYTOPLASM
expression_system_vector_type: PLASMID
expression_system_plasmid: PET28B(+)
Authors : Starks, C.M., Back, K., Chappell, J., Noel, J.P.
Keywords : ISOPRENOID SYNTHASE, 5-EPI-ARISTOLOCHENE SYNTHASE, NATURAL PRODUCTS BIOSYNTHESIS, ISOPRENOID CYCLASE
Exp. method : X-RAY DIFFRACTION ( 2.15 Å )
Citation :

Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase.

Starks, C.M.,Back, K.,Chappell, J.  et al.
(1997)  Science  277 : 1815 - 1820

PubMed: 9295271
DOI: 10.1126/science.277.5333.1815

Expression of a Plant Sesquiterpene Cyclase Gene in Escherichia Coli

Back, K.,Yin, S.,Chappell, J.
(1994)  Arch.Biochem.Biophys.  315 : 527

Purification and Characterization of an Inducible Sesquiterpene Cyclase from Elicitor-Treated Tobacco Cell Suspension Cultures

Vogeli, U.,Freeman, J.W.,Chappell, J.
(1993)  Plant Physiol.  93 : 182

Chain : A
UniProt : Q40577 (5EAS_TOBAC)
Reaction : (2E,6E)-farnesyl diphosphate = (+)-5- epiaristolochene + diphosphate.