Brand  (β version)

PDB ID: 5EAT

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Ligands
Code Name Style Show Link
FHP 1-hydroxy-3,7,11-trimethyldodeca-2,6,10-triene phosphonic acid PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 5EAT   PDBj   RCSB PDB   PDBe
Header : ISOPRENOID SYNTHASE
Title : 5-EPI-ARISTOLOCHENE SYNTHASE FROM NICOTIANA TABACUM WITH SUBSTRATE ANALOG FARNESYL HYDROXYPHOSPHONATE
Release Data : 1997-11-12
Compound :
mol_id molecule chains synonym
1 5-EPI-ARISTOLOCHENE SYNTHASE A 5-EPI-ARISTOLOCHENE CYCLASE
Source :
mol_id organism_scientific organism_common expression_system
1 Nicotiana tabacum  (taxid:4097) Common tobacco Escherichia coli BL21(DE3)  (taxid:469008)
strain: NK326
cell_line: BL21
expression_system_strain: BL21 (DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PET28B(+)
Authors : Starks, C.M., Back, K., Chappell, J., Noel, J.P.
Keywords : ISOPRENOID SYNTHASE, ISOPRENOID CYCLASE, 5-EPI-ARISTOLOCHENE SYNTHASE, ISOPRENOID BIOSYNTHESIS, NATURAL PRODUCTS BIOSYNTHESIS
Exp. method : X-RAY DIFFRACTION ( 2.8 Å )
Citation :

Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase.

Starks, C.M.,Back, K.,Chappell, J.  et al.
(1997)  Science  277 : 1815 - 1820

PubMed: 9295271
DOI: 10.1126/science.277.5333.1815

Expression of a Plant Sesquiterpene Cyclase Gene in Escherichia Coli

Back, K.,Yin, S.,Chappell, J.
(1994)  Arch.Biochem.Biophys.  315 : 527

Purification and Characterization of an Inducible Sesquiterpene Cyclase from Elicitor-Treated Tobacco Cell Suspension Cultures

Vogeli, U.,Freeman, J.W.,Chappell, J.
(1990)  Plant Physiol.  93 : 182

Reaction

Chain : A
UniProt : Q40577 (5EAS_TOBAC)
Reaction: EC: Evidence:
Physiological Direction:
(2E,6E)-farnesyl diphosphate = (+)-5-epi-aristolochene + diphosphate 4.2.3.61 PubMed:16375847, Ref.3
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