Brand  (β version)

PDB ID: 5E97

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Ligands
Code Name Style Show Link
CL Chloride ion PoSSuM
NPO P-nitrophenol PoSSuM
BDP Beta-D-glucopyranuronic acid PoSSuM
NDG 2-acetamido-2-deoxy-alpha-D-glucopyranose PoSSuM
: Polysaccharide
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
NAG 2-acetamido-2-deoxy-beta-D-glucopyranose
Modification
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Structure summary

Code : 5E97   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Glycoside Hydrolase ligand structure 1
Release Data : 2015-11-18
Compound :
mol_id molecule chains synonym
1 Heparanase A Endo-glucoronidase,Heparanase-1,Hpa1
ec: 3.2.1.166
fragment: residues 158-543
mol_id molecule chains synonym
2 Heparanase B Endo-glucoronidase,Heparanase-1,Hpa1
ec: 3.2.1.166
fragment: residues 36-109
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Trichoplusia ni  (taxid:7111)
gene: HPSE, HEP, HPA, HPA1, HPR1, HPSE1, HSE1
expression_system_common: Cabbage looper
mol_id organism_scientific organism_common expression_system
2 Homo sapiens  (taxid:9606) Human Trichoplusia ni  (taxid:7111)
gene: HPSE, HEP, HPA, HPA1, HPR1, HPSE1, HSE1
expression_system_common: Cabbage looper
Authors : Wu, L., Davies, G.J.
Keywords : glycoside hydrolase, ligand 1, protein, sugar, hydrolase
Exp. method : X-RAY DIFFRACTION ( 1.63 Å )
Citation :

Structural characterization of human heparanase reveals insights into substrate recognition.

Wu, L.,Viola, C.M.,Brzozowski, A.M.  et al.
(2015)  Nat.Struct.Mol.Biol.  22 : 1016 - 1022

PubMed: 26575439
DOI: 10.1038/nsmb.3136

Reaction

Chain : B
UniProt : Q9Y251 (HPSE_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
endohydrolysis of (1->4)-beta-D-glycosidic bonds of heparan sulfate chains in heparan sulfate proteoglycan. 3.2.1.166 PubMed:11973358, PubMed:12213822, PubMed:12927802, PubMed:20181948, PubMed:20561914, PubMed:20634491
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