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Ligands
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AG Silver ion
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Code : 5A4G   PDBj   RCSB PDB   PDBe
Header : METAL BINDING PROTEIN
Title : NMR structure of a 180 residue construct encompassing the N-terminal metal-binding site and the membrane proximal domain of SilB from Cupriavidus metallidurans CH34
Release Data : 2016-05-18
Compound :
mol_id molecule chains synonym
1 SILB, SILVER EFFLUX PROTEIN, MFP COMPONENT OF THE THREE COMPONENTS PROTON ANTIPORTER METAL EFFLUX SYSTEM A AG_SILBNM2
fragment: UNP RESIDUES 36-123,344-426
other_details: THE CONSTRUCT IS NUMBERED 1-180,1 BEING THE N-TERMINAL METHIONINE. THE TWO PARTS OF THE MEMBRANE PROXIMAL DOMAIN ARE LINKED BY GS LOOP (RESIDUES 90 AND 91, CONSTRUCT NUMBERING)
Source :
mol_id organism_scientific expression_system
1 CUPRIAVIDUS METALLIDURANS  (taxid:266264) ESCHERICHIA COLI  (taxid:469008)
strain: CH34
atcc: 43123
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PET28
Authors : Bersch, B., Urbina Fernandez, P., Vandenbussche, G.
Keywords : METAL BINDING PROTEIN, MEMBRANE FUSION PROTEIN, METAL SITE, SILVER, RESISTANCE NODULATION CELL DIVISION, RND
Exp. method : SOLUTION NMR
Citation :

Structural and Functional Investigation of the Ag+/Cu+-Binding Domains of the Periplasmic Adaptor Protein Silb from Cupriavidus Metallidurans Ch34.

Urbina, P.,Bersch, B.,De Angelis, F.  et al.
(2016)  Biochemistry  55 : 2883

PubMed: 27145046
DOI: 10.1021/ACS.BIOCHEM.6B00022

Chain : A
UniProt : Q58AF3 (Q58AF3_CUPMC)
Reaction : -