Brand  (β version)

PDB ID: 5XXK

Hetero Atom Contents

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Ligands
Code Name Style Show Link
Non-standard Residues
Code Name Show
THC N-methylcarbonylthreonine
0EH (2r)-2-amino-2-methylnonanoic acid
6CW 6-chloro-L-tryptophan
MK8 2-methyl-L-norleucine
Glycosylation
Code Name Emphasize
Modification
Code Name Show
NH2 Amino group

Structure summary

Code : 5XXK   PDBj   RCSB PDB   PDBe
Header : ONCOPROTEIN/INHIBITOR
Title : Structure-activity studies of Mdm2/Mdm4-binding stapled peptides comprising non-natural amino acids
Release Data : 2017-12-27
Compound :
mol_id molecule chains synonym
1 E3 ubiquitin-protein ligase Mdm2 A,B Double minute 2 protein,Hdm2,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2
ec: 2.3.2.27
fragment: UNP residues 6-125
mutation: M62A
mol_id molecule chains
2 Hydrocarbon stapled peptide THC-SER-PHE-0EH-GLU-TYR-6CW-ALA-LEU-LEU-MK8-NH2 C,D
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli BL21  (taxid:511693)
gene: MDM2
expression_system_strain: BL21
expression_system_vector_type: plasmid
expression_system_plasmid: PGEX 6.1
mol_id organism_scientific
2 Phage display vector pTDisp  (taxid:279974)
synthetic: yes
Authors : Brown, C.J.
Keywords : E3 ubiqutin ligase, ONCOPROTEIN, ONCOPROTEIN-INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 1.66 Å )
Citation :

Structure-activity studies of Mdm2/Mdm4-binding stapled peptides comprising non-natural amino acids.

Chee, S.M.Q.,Wongsantichon, J.,Siau, J.  et al.
(2017)  PLoS ONE  12 : e0189379 - e0189379

PubMed: 29228061
DOI: 10.1371/journal.pone.0189379

Reaction

Chain : A, B
UniProt : Q00987 (MDM2_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. 2.3.2.27 PubMed:12821780
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