Brand  (β version)

PDB ID: 5WKC

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Ligands
Code Name Style Show Link
MG Magnesium ion PoSSuM
PXD 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-C]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide PoSSuM
FAD Flavin-adenine dinucleotide PoSSuM
F50 Ethaneperoxoic acid PoSSuM
AUJ 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate PoSSuM
TP9 (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate PoSSuM
Non-standard Residues
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SME Methionine sulfoxide
Glycosylation
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Modification
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Structure summary

Code : 5WKC   PDBj   RCSB PDB   PDBe
Header : STRUCTURAL PROTEIN,Transferase
Title : Saccharomyces cerevisiae acetohydroxyacid synthase in complex with the herbicide penoxsulam
Release Data : 2018-02-14
Compound :
mol_id molecule chains synonym
1 Acetolactate synthase catalytic subunit, mitochondrial A,B,E Acetohydroxy-acid synthase catalytic subunit,ALS
ec: 2.2.1.6
fragment: UNP residues 58-687
mol_id molecule chains synonym
2 Acetolactate synthase catalytic subunit, mitochondrial D Acetohydroxy-acid synthase catalytic subunit,ALS
ec: 2.2.1.6
fragment: UNP residues 58-687
Source :
mol_id organism_scientific organism_common expression_system
1 Saccharomyces cerevisiae S288c  (taxid:559292) Baker's yeast Escherichia coli  (taxid:562)
strain: ATCC 204508 / S288c
gene: ILV2, SMR1, YMR108W, YM9718.07
mol_id organism_scientific organism_common expression_system
2 Saccharomyces cerevisiae S288c  (taxid:559292) Baker's yeast Escherichia coli  (taxid:562)
strain: ATCC 204508 / S288c
gene: ILV2, SMR1, YMR108W, YM9718.07
Authors : Guddat, W.L., Lonhienne, G.T.
Keywords : AHAS, acetohydroxyacid synthase; ScAHAS; thiamine diphosphate; flavin adenine dinucleotide; penoxsulam; aminoethenethiol diphosphate; peracetate., STRUCTURAL PROTEIN, Transferase
Exp. method : X-RAY DIFFRACTION ( 2.334 Å )
Citation :

Structural insights into the mechanism of inhibition of AHAS by herbicides.

Lonhienne, T.,Garcia, M.D.,Pierens, G.  et al.
(2018)  Proc. Natl. Acad. Sci. U.S.A.  115 : E1945 - E1954

PubMed: 29440497
DOI: 10.1073/pnas.1714392115

Reaction

Chain : D
UniProt : P07342 (ILVB_YEAST)
Reaction: EC: Evidence:
Physiological Direction:
2 pyruvate + H(+) = (2S)-2-acetolactate + CO2 2.2.1.6 PubMed:10213630
left-to-right
2-oxobutanoate + pyruvate + H(+) = (S)-2-ethyl-2-hydroxy-3- oxobutanoate + CO2 2.2.1.6 PubMed:10213630
left-to-right
Cofactor: Evidence: Note:
Mg(2+) ECO:0000250
Binds 1 Mg(2+) ion per subunit.
thiamine diphosphate ECO:0000250
Binds 1 thiamine pyrophosphate per subunit.