Brand  (β version)

PDB ID: 5VYU

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Ligands
Code Name Style Show Link
1YA N-{4-[{[(6s)-2-amino-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}(formyl)amino]benzoyl}-L-glutamic acid PoSSuM
GDP Guanosine-5'-diphosphate PoSSuM
JB2 Gdp-perosamine PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 5VYU   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of the WbkC N-formyltransferase from Brucella melitensis in complex with GDP-perosaminea and N-10-formyltetrahydrofolate
Release Data : 2017-07-05
Compound :
mol_id molecule chains
1 Gdp-mannose 4,6-dehydratase / gdp-4-amino-4,6-dideoxy-d-mannose formyltransferase A,B
ec: 2.1.2.-,4.2.1.47
mutation: D78A, F142A
Source :
mol_id organism_scientific expression_system
1 Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)  (taxid:224914) Escherichia coli  (taxid:562)
strain: 16M / ATCC 23456 / NCTC 10094
gene: BMEI1418
expression_system_strain: Rosetta2(DE3)
Authors : Riegert, A.S., Chantigian, D.P., Thoden, J.B., Holden, H.M.
Keywords : deoxysugar, brucellosis, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.20 Å )
Citation :

Biochemical Characterization of WbkC, an N-Formyltransferase from Brucella melitensis.

Riegert, A.S.,Chantigian, D.P.,Thoden, J.B.  et al.
(2017)  Biochemistry  56 : 3657 - 3668

PubMed: 28636341
DOI: 10.1021/acs.biochem.7b00494

Reaction

Chain : A, B
UniProt : F8WJP6 (WBKC_BRUME)
Reaction: EC: Evidence:
Physiological Direction:
(6R)-10-formyltetrahydrofolate + GDP-alpha-D-perosamine = (6S)-5,6,7,8-tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine + H(+) 2.1.2.14 PubMed:28636341
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