Brand  (β version)

PDB ID: 5VK0

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Ligands
Code Name Style Show Link
Non-standard Residues
Code Name Show
9E7 N~6~-(sulfanylmethyl)-L-lysine
Glycosylation
Code Name Emphasize
Modification
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ACE Acetyl group
NH2 Amino group

Structure summary

Code : 5VK0   PDBj   RCSB PDB   PDBe
Header : LIGASE/LIGASE INHIBITOR
Title : Crystal structure of human MDM2 in complex with a 12-mer lysine-cysteine side chain dithiocarbamate stapled peptide inhibitor PMI
Release Data : 2018-04-25
Compound :
mol_id molecule chains synonym
1 E3 ubiquitin-protein ligase Mdm2 A,C,E,G,I,K,M,O,Q,S,U,W Double minute 2 protein,Hdm2,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2
ec: 2.3.2.27
fragment: residues 25-109
mol_id molecule chains
2 Lysine-cysteine side chain dithiocarbamate stapled peptide inhibitor PMI B,D,F,H,J,L,N,P,R,T,V,X
Source :
mol_id organism_scientific organism_common
1 Homo sapiens  (taxid:9606) Human
synthetic: yes
mol_id organism_scientific
2 Synthetic construct  (taxid:32630)
synthetic: yes
Authors : Tolbert, W.D., Gohain, N., Pazgier, M.
Keywords : MDM2-PEPTIDE INHIBITOR COMPLEX, ONCOPROTEIN, HOST-VIRUS INTERACTION, LIGASE, METAL-BINDING, NUCLEUS, PHOSPHOPROTEIN, PROTO-ONCOGENE, UBL CONJUGATION PATHWAY, ZINC-FINGER, stapled peptide, LIGASE-LIGASE INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 1.80 Å )
Citation :

Dithiocarbamate-inspired side chain stapling chemistry for peptide drug design.

Li, X.,Tolbert, W.D.,Hu, H.G.  et al.
(2019)  Chem Sci  10 : 1522 - 1530

PubMed: 30809370
DOI: 10.1039/c8sc03275k

Reaction

Chain : A, C, E, G, I, K, M, O, Q, S, U, W
UniProt : Q00987 (MDM2_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. 2.3.2.27 PubMed:12821780
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