Brand  (β version)

PDB ID: 5V0A

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Ligands
Code Name Style Show Link
D5M 2'-deoxyadenosine-5'-monophosphate PoSSuM
MN Manganese (II) ion PoSSuM
NA Sodium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 5V0A   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/DNA
Title : Crystal structure of human exonuclease 1 Exo1 (D225A) in complex with 5' recessed-end DNA (rVIII)
Release Data : 2017-05-24
Compound :
mol_id molecule chains synonym
1 Exonuclease 1 Z hExo1, Exonuclease I, hExoI
ec: 3.1.-.-
fragment: UNP residues 1-352
mutation: D225A
mol_id molecule chains
2 DNA (5'-D(*CP*GP*CP*TP*AP*GP*TP*CP*GP*TP*CP*AP*T)-3') A
mol_id molecule chains
3 DNA (5'-D(P*CP*GP*AP*CP*TP*AP*GP*CP*G)-3') B
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: EXO1, EXOI, HEX1
mol_id organism_scientific
2 Synthetic construct  (taxid:32630)
synthetic: yes
mol_id organism_scientific
3 Synthetic construct  (taxid:32630)
synthetic: yes
Authors : Shi, Y., Beese, L.S.
Keywords : exonuclease, endonuclease, HYDROLASE-DNA complex
Exp. method : X-RAY DIFFRACTION ( 2.38 Å )
Citation :

Interplay of catalysis, fidelity, threading, and processivity in the exo- and endonucleolytic reactions of human exonuclease I.

Shi, Y.,Hellinga, H.W.,Beese, L.S.
(2017)  Proc. Natl. Acad. Sci. U.S.A.  114 : 6010 - 6015

PubMed: 28533382
DOI: 10.1073/pnas.1704845114

Reaction

Chain : Z
UniProt : Q9UQ84 (EXO1_HUMAN)
Reaction : -
Cofactor: Evidence: Note:
Mg(2+) ECO:0000250
Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.