Brand  (β version)

PDB ID: 5TUI

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Ligands
Code Name Style Show Link
SO4 Sulfate ion PoSSuM
FAD Flavin-adenine dinucleotide PoSSuM
Non-standard Residues
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Structure summary

Code : 5TUI   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal structure of tetracycline destructase Tet(50) in complex with chlortetracycline
Release Data : 2017-05-10
Compound :
mol_id molecule chains
1 Tetracycline destructase Tet(50) A,B
Source :
mol_id organism_scientific expression_system
1 Uncultured bacterium  (taxid:77133) Escherichia coli BL21(DE3)  (taxid:469008)
expression_system_vector_type: plasmid
expression_system_plasmid: pET28b(+)
Authors : Park, J., Tolia, N.H.
Keywords : FAD-binding, tetracycline-inactivating, oxidoreductase activity, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.750 Å )
Citation :

Plasticity, dynamics, and inhibition of emerging tetracycline resistance enzymes.

Park, J.,Gasparrini, A.J.,Reck, M.R.  et al.
(2017)  Nat. Chem. Biol.  13 : 730 - 736

PubMed: 28481346
DOI: 10.1038/nchembio.2376

Reaction

Chain : A, B
UniProt : A0A059WYP6 (TET50_UNKP)
Reaction: EC: Evidence:
Physiological Direction:
a tetracycline + H(+) + NADPH + O2 = a (1S,10aS)-3-(CONH2)-1- (Me2N)-3,3a,4,6-(HO)4-2,5-dioxo-1H,10aH,11H,11aH- cyclopenta[b]anthracene + CO + H2O + NADP(+) - PubMed:26097034, PubMed:28481346
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7-chlorotetracycline + H(+) + NADPH + O2 = (1S,10S,10aS)-3- (CONH2)-9-Cl-1-(Me2N)-3,3a,4,10-(HO)4-10-Me-2,5-dioxo- 1H,10aH,11H,11aH-cyclopenta[b]anthracen-6-olate + CO + H2O + NADP(+) - PubMed:28481346
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