Brand  (β version)

PDB ID: 5T2T

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Ligands
Code Name Style Show Link
MN Manganese (II) ion PoSSuM
0N8 (2z)-4-[1-benzyl-4-(4-chlorobenzyl)piperidin-4-yl]-2-hydroxy-4-oxobut-2-enoic acid PoSSuM
GOL Glycerol PoSSuM
CL Chloride ion PoSSuM
NA Sodium ion PoSSuM
Non-standard Residues
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Structure summary

Code : 5T2T   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of Lymphocytic choriomeningitis mammarenavirus endonuclease bound to compound L742001
Release Data : 2017-09-13
Compound :
mol_id molecule chains synonym
1 RNA-directed RNA polymerase L A,B Protein L
ec: 2.7.7.48
Source :
mol_id organism_scientific expression_system
1 Lymphocytic choriomeningitis mammarenavirus  (taxid:11623) Escherichia coli  (taxid:562)
gene: L, KUE_IGS310002
Authors : Saez-Ayala, M., Yekwa, E.L., Canard, B., Alvarez, K., Ferron, F.
Keywords : mammarenavirus, LCMV, endonuclease, inhibitor, transferase
Exp. method : X-RAY DIFFRACTION ( 1.967 Å )
Citation :

Crystal structures ofLymphocytic choriomeningitis virusendonuclease domain complexed with diketo-acid ligands.

Saez-Ayala, M.,Yekwa, E.L.,Carcelli, M.  et al.
(2018)  IUCrJ  5 : 223 - 235

PubMed: 29765612
DOI: 10.1107/S2052252518001021

Reaction

Chain : A, B
UniProt : A0A059U382 (A0A059U382_9VIRU)
Reaction: EC: Evidence:
Physiological Direction:
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate 2.7.7.48 ARBA:ARBA00048744, HAMAP-Rule:MF_04086, PIRNR:PIRNR000836
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Cofactor: Evidence: Note:
Mn(2+) ECO:0000256 | HAMAP-Rule:MF_04086
ECO:0000256 | HAMAP-Rule:MF_04086
For polymerase activity.
Mn(2+) ECO:0000256 | HAMAP-Rule:MF_04086
For endonuclease activity. Binds 2 Mn(2+) ions in the active site. The divalent metal ions are crucial for catalytic activity.