Brand  (β version)

PDB ID: 5SWM

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Ligands
Code Name Style Show Link
NA Sodium ion PoSSuM
CL Chloride ion PoSSuM
PEG Di(hydroxyethyl)ether PoSSuM
GOL Glycerol PoSSuM
Non-standard Residues
Code Name Show
GF2 2'-deoxy-2'-fluoroguanosine 5'-(dihydrogen phosphate)
AF2 2'-deoxy-2'-fluoroadenosine 5'-(dihydrogen phosphate)
CFZ 2'-deoxy-2'-fluorocytidine 5'-(dihydrogen phosphate)
UFT 2'-deoxy-2'-fluorouridine 5'-(dihydrogen phosphate)
BRU 5-bromo-2'-deoxyuridine-5'-monophosphate
Glycosylation
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Modification
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Structure summary

Code : 5SWM   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/RNA/DNA
Title : BACILLUS HALODURANS RNASE H MUTANT D132N IN COMPLEX WITH 12-MER FRNA/DNA HYBRID
Release Data : 2016-09-21
Compound :
mol_id molecule chains synonym
1 Ribonuclease H A,B RNase H
ec: 3.1.26.4
mutation: D132N
mol_id molecule chains
2 RNA (12-MER) C
mol_id molecule chains
3 DNA (12-MER) D
Source :
mol_id organism_scientific expression_system
1 Bacillus halodurans  (taxid:86665) BACILLUS HALODURANS  (taxid:86665)
gene: rnhA
expression_system_strain: BL21 PLYSS
expression_system_plasmid: PET15B
mol_id organism_scientific
2 Synthetic construct  (taxid:32630 )
synthetic: yes
mol_id organism_scientific
3 Synthetic construct  (taxid:32630 )
synthetic: yes
Authors : Pallan, P.S., Egli, M.
Keywords : RNASE H, RNA/DNA HYBRID, HYDROLASE-RNA-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 1.50 Å )
Citation :

Limits of RNA 2'-OH Mimicry by Fluorine: Crystal Structure of Bacillus halodurans RNase H Bound to a 2'-FRNA:DNA Hybrid.

Pallan, P.S.,Prakash, T.P.,de Leon, A.R.  et al.
(2016)  Biochemistry  55 : 5321 - 5325

PubMed: 27611889
DOI: 10.1021/acs.biochem.6b00849

Reaction

Chain : A, B
UniProt : Q9KEI9 (RNH1_HALH5)
Reaction: EC: Evidence:
Physiological Direction:
Endonucleolytic cleavage to 5'-phosphomonoester. 3.1.26.4 -
-
Cofactor: Evidence: Note:
Mg(2+) - Binds 2 metal ions per subunit. Manganese or magnesium.