Brand  (β version)

PDB ID: 5OGL

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Ligands
Code Name Style Show Link
9UB [(2~{S},3~{R},4~{R},5~{S},6~{R})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]methyl-[oxidanyl-[(2~{z},6~{z},10~{z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl]oxy-phosphinic acid PoSSuM
MN Manganese (II) ion PoSSuM
NA Sodium ion PoSSuM
Non-standard Residues
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PPN Para-nitrophenylalanine
Glycosylation
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Modification
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Structure summary

Code : 5OGL   PDBj   RCSB PDB   PDBe
Header : MEMBRANE PROTEIN
Title : Structure of bacterial oligosaccharyltransferase PglB in complex with an acceptor peptide and an lipid-linked oligosaccharide analog
Release Data : 2017-10-25
Compound :
mol_id molecule chains synonym
1 Undecaprenyl-diphosphooligosaccharide--protein glycotransferase A Protein glycosylation B
ec: 2.4.99.19
mutation: K2E, C17A, C30A, A108T, C360L, N535Q, Q536K, K549P, D550N, F553I, N556P, A600P, A602D, T606K, T607Q, V610I, M611T, I619S, F622Y, A624S, V627I, A630N, F663Y, F670Y
mol_id molecule chains
2 Substrate mimicking peptide B
Source :
mol_id organism_scientific expression_system
1 Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060)  (taxid:306263) Escherichia coli BL21(DE3)  (taxid:469008)
strain: RM2100 / D67 / ATCC BAA-1060
gene: pglB, Cla_1253
mol_id organism_scientific
2 Campylobacter jejuni  (taxid:197)
synthetic: yes
Authors : Napiorkowska, M., Boilevin, J., Sovdat, T., Darbre, T., Reymond, J.-L., Aebi, M., Locher, K.P.
Keywords : Oligosaccharyltransferase, Complex, Protein N-glycosylation, Bacteria, MEMBRANE PROTEIN
Exp. method : X-RAY DIFFRACTION ( 2.700 Å )
Citation :

Molecular basis of lipid-linked oligosaccharide recognition and processing by bacterial oligosaccharyltransferase.

Napiorkowska, M.,Boilevin, J.,Sovdat, T.  et al.
(2017)  Nat. Struct. Mol. Biol.  24 : 1100 - 1106

PubMed: 29058712
DOI: 10.1038/nsmb.3491

Reaction

Chain : A
UniProt : B9KDD4 (PGLB_CAMLR)
Reaction: EC: Evidence:
Physiological Direction:
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine = tritrans,heptacis-undecaprenyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D- glycosyl linkage to protein L-asparagine. 2.4.99.19 PubMed:21677752, PubMed:24149797
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