Brand  (β version)

PDB ID: 5OBP

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Ligands
Code Name Style Show Link
9QQ 6-hydroxybenzimidazolyl-norcobamide PoSSuM
BEN Benzamidine PoSSuM
GOL Glycerol PoSSuM
SF4 Iron/sulfur cluster PoSSuM
Non-standard Residues
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Structure summary

Code : 5OBP   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : PCE reductive dehalogenase from S. multivorans with 6-hydroxybenzimidazolyl norcobamide cofactor
Release Data : 2018-02-14
Compound :
mol_id molecule chains
1 Tetrachloroethene reductive dehalogenase catalytically active subunit A,B
Source :
mol_id organism_scientific
1 Sulfurospirillum multivorans  (taxid:66821)
Authors : Keller, S., Kunze, C., Bommer, M., Paetz, C., Menezes, R.C., Svatos, A., Dobbek, H., Schubert, T.
Keywords : organohalide respiration, anaerobic crystallisation, cobalamin, oxidoreductase
Exp. method : X-RAY DIFFRACTION ( 1.593 Å )
Citation :

Selective Utilization of Benzimidazolyl-Norcobamides as Cofactors by the Tetrachloroethene Reductive Dehalogenase of Sulfurospirillum multivorans.

Keller, S.,Kunze, C.,Bommer, M.  et al.
(2018)  J. Bacteriol.  200

PubMed: 29378885
DOI: 10.1128/JB.00584-17

Reaction

Chain : A, B
UniProt : O68252 (PCEA_SULMU)
Reaction: EC: Evidence:
Physiological Direction:
trichloroethene + chloride + A + H(+) = tetrachloroethene + AH2 1.21.99.5 PubMed:11976751, PubMed:12420164, PubMed:24433392, PubMed:28671181, PubMed:8663199
right-to-left
trichloroethene + AH2 = (Z)-1,2-dichloroethene + chloride + A + H(+) - PubMed:11976751, PubMed:12420164, PubMed:24433392, PubMed:28671181, PubMed:8663199
left-to-right
Cofactor: Evidence: Note:
[4Fe-4S] cluster ECO:0000269 | PubMed:28671181
ECO:0000269 | PubMed:29378885
ECO:0000269 | PubMed:8663199
Binds 2 [4Fe-4S] clusters.
corrinoid ECO:0000269 | PubMed:11976751
ECO:0000269 | PubMed:12420164
ECO:0000269 | PubMed:24433392
ECO:0000269 | PubMed:28671181
ECO:0000269 | PubMed:8663199
ECO:0000269 | Ref.6
The corrinoid cofactor is norpseudovitamin B12 (norpseudo-B12), a natural B12 cofactor that lacks a characteristic methyl group of the cobamide structure (PubMed:24433392, PubMed:28671181, Ref.6). In vitro, can function with singly substituted benzimidazolyl-norcobamides cofactors (PubMed:29378885).