Brand  (β version)

PDB ID: 5O7E

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Ligands
Code Name Style Show Link
9NB ~{N}-(4-ethanoylphenyl)-2-sulfanyl-ethanamide PoSSuM
CA Calcium ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 5O7E   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal structure of the peptidase domain of collagenase H from Clostridium histolyticum in complex with N-aryl mercaptoacetamide-based inhibitor
Release Data : 2018-01-31
Compound :
mol_id molecule chains synonym
1 ColH protein A Collagenase
other_details: Non-aligned residues at N-terminus were not ordered in crystal. GT = position 1-2, remainder of tag after cleavage LDK ... PNEG = position 3 -394, sequence of peptidase domain of ColH, corresponding to L331-G721 of Uniprot entry Q46085
Source :
mol_id organism_scientific expression_system
1 Hathewaya histolytica  (taxid:1498) Escherichia coli  (taxid:562)
gene: colH
expression_system_strain: NiCo21 (DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET15b
Authors : Schoenauer, E., Brandstetter, H.
Keywords : HYDROLASE-INHIBITOR COMPLEX, GLUZINCIN, METALLOPROTEASE, collagenase, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.870 Å )
Citation :

Discovery of a Potent Inhibitor Class with High Selectivity toward Clostridial Collagenases.

Schonauer, E.,Kany, A.M.,Haupenthal, J.  et al.
(2017)  J. Am. Chem. Soc.  139 : 12696 - 12703

PubMed: 28820255
DOI: 10.1021/jacs.7b06935

Reaction

Chain : A
UniProt : Q46085 (COLH_HATHI)
Reaction: EC: Evidence:
Physiological Direction:
Digestion of native collagen in the triple helical region at Xaa-|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3'. 3.4.24.3 PubMed:24125730, PubMed:3002446, PubMed:10217773, PubMed:18937627, PubMed:28820255
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