Brand  (β version)

PDB ID: 5O5E

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Ligands
Code Name Style Show Link
9LH Tunicamycin PoSSuM
P6L (2s)-3-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-2-[(6e)-hexadec-6-enoyloxy]propyl (8e)-octadec-8-enoate PoSSuM
UNL Unknown ligand PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 5O5E   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of human UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) (V264G mutant) in complex with tunicamycin
Release Data : 2018-02-28
Compound :
mol_id molecule chains synonym
1 UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase A GlcNAc-1-P transferase,GPT,N-acetylglucosamine-1-phosphate transferase
ec: 2.7.8.15
mutation: V264G
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Spodoptera frugiperda  (taxid:7108)
gene: DPAGT1, DPAGT2
expression_system_common: Fall armyworm
expression_system_vector_type: BACULOVIRUS
expression_system_plasmid: PFB-LIC-BSE
Authors : Pike, A.C.W., Dong, Y.Y., Chu, A., Tessitore, A., Goubin, S., Dong, L., Mukhopadhyay, S., Mahajan, P., Chalk, R., Berridge, G., Wang, D., Kupinska, K., Belaya, K., Beeson, D., Burgess-Brown, N., Edwards, A.M., Arrowsmith, C.H., Bountra, C., Carpenter, E.P., Structural Genomics Consortium (SGC)
Keywords : Protein glycosylation, integral membrane protein, PNPT, congenital myasthenic syndrome, antibiotic, Structural Genomics, Structural Genomics Consortium, SGC, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 3.40 Å )
Citation :

Structures of DPAGT1 Explain Glycosylation Disease Mechanisms and Advance TB Antibiotic Design.

Dong, Y.Y.,Wang, H.,Pike, A.C.W.  et al.
(2018)  Cell  175 : 1045 - 1058.e16

PubMed: 30388443
DOI: 10.1016/j.cell.2018.10.037

Reaction

Chain : A
UniProt : Q9H3H5 (GPT_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
a dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = N- acetyl-alpha-D-glucosaminyl-diphosphodolichol + UMP 2.7.8.15 PubMed:29459785, PubMed:30388443, PubMed:9451016
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