Het-PDB Navi2 (PDB: 5NXQ)

The number of atoms exceeds 100,000.
So, it can not be displayed here.

Select unit:

Select hetatm:

information

centroid:

interaction residue:

Select chain: Sequence Hide other chain(s)

Data format:

Color scheme of protein:

Ligands
Code	Name		Style	Show	Link
GOL	Glycerol				PoSSuM

Non-standard Residues
Code	Name	Show

Glycosylation
Code	Name	Emphasize

Modification
Code	Name	Show
9FZ	(2~{S})-2-azanyl-5-(4-methyl-1,2,3-triazol-1-yl)pentanoic acid
9G2	(2~{S})-2-azanyl-5-(4-ethyl-1,2,3-triazol-1-yl)pentanoic acid

Download interaction data: 5NXQ

Code :

5NXQ PDBj RCSB PDB PDBe

Header :

REPLICATION

Title :

Crystal structure of the carboxy-terminal domain of yeast Ctf4 bound to a stapled Sld5 CIP

Release Data :

2017-08-30

Compound :

mol_id	molecule	chains	synonym
1	DNA polymerase alpha-binding protein	A,B,C	Chromosome replication protein CHL15,Chromosome transmission fidelity protein 4,Protein POB1
	fragment: UNP residues 471-927 other_details: Ctf4-CTD (amino acids 427 to 971; C-end) was expressed and purified with an N-terminal Histag followed by a TEV protease cleavage site.

mol_id	molecule	chains
2	MET-ASP-ILE-UA1-ILE-ASP-ASP-ILE-LEU-UA2-GLU-LEU-ASP-LYS-GLU	D,E
	other_details: The molecule is a stapled version of the wild-type Sld5 CIP. it was produced by replacing amino acids 4 and 11 with two unnatural amino acids (UA1 and UA2) containing azide moieties in their side chains, and reacting the resulting diazido peptide with a bis-triazole dialkynyl linker, via a Cu(I)-catalysed click chemistry reaction.

Source :

mol_id	organism_scientific	organism_common	expression_system
1	Saccharomyces cerevisiae (taxid:4932)	Baker's yeast	Escherichia coli (taxid:562)
	gene: CTF4, CHL15, POB1, YPR135W, P9659.7 expression_system_vector_type: plasmid expression_system_plasmid: pRSF-Duet1

mol_id	organism_scientific
2	Saccharomyces cerevisiae (taxid:4932)
	synthetic: yes

Authors :

Wu, Y., Pellegrini, L.

Keywords :

DNA replication, adaptor protein, beta-propeller domain, replication

Exp. method :

X-RAY DIFFRACTION ( 2.413 Å )

Citation :

Targeting the Genome-Stability Hub Ctf4 by Stapled-Peptide Design.

Wu, Y.,Villa, F.,Maman, J. et al.
(2017) Angew. Chem. Int. Ed. Engl. 56 : 12866 - 12872

A Ctf4 trimer couples the CMG helicase to DNA polymerase alpha in the eukaryotic replisome.

Simon, A.C.,Zhou, J.C.,Perera, R.L. et al.
(2014) Nature 510 : 293 - 297

PDB ID: 5NXQ