Brand  (β version)

PDB ID: 5NU5

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Ligands
Code Name Style Show Link
99E ~{N}-[[2,8-bis(oxidanyl)naphthalen-1-yl]methyl]-4-ethanoyl-3-ethyl-5-methyl-1~{H}-pyrrole-2-carboxamide PoSSuM
EDO 1,2-ethanediol PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 5NU5   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of the human bromodomain of EP300 bound to the inhibitor XDM-CBP
Release Data : 2017-08-16
Compound :
mol_id molecule chains synonym
1 Histone acetyltransferase p300 A,B p300 HAT,E1A-associated protein p300
ec: 2.3.1.48
fragment: bromodomain
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli BL21(DE3)  (taxid:469008)
gene: EP300, P300
expression_system_vector_type: plasmid
expression_system_plasmid: pNIC28-Bsa4
Authors : Huegle, M., Wohlwend, D.
Keywords : bromodomain, protein-inhibitor complex, epigenetics, EP300, transferase
Exp. method : X-RAY DIFFRACTION ( 1.6000 Å )
Citation :

Beyond the BET Family: Targeting CBP/p300 with 4-Acyl Pyrroles.

Hugle, M.,Lucas, X.,Ostrovskyi, D.  et al.
(2017)  Angew. Chem. Int. Ed. Engl.  56 : 12476 - 12480

PubMed: 28766825
DOI: 10.1002/anie.201705516

Reaction

Chain : A, B
UniProt : Q09472 (EP300_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+) 2.3.1.48 PubMed:14645221, PubMed:21131905, PubMed:23415232, PubMed:23934153, PubMed:25818647, PubMed:8945521
left-to-right
L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+) - PubMed:15448695, PubMed:15653507, PubMed:16285960, PubMed:17761950, PubMed:22084251, PubMed:24120661, PubMed:26990986, PubMed:30197302, PubMed:32561715
left-to-right PubMed:15653507, PubMed:16285960, PubMed:15448695, PubMed:30197302, PubMed:32561715
N(6)-methyl-L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-N(6)- methyl-L-lysyl-[histone] + CoA + H(+) - PubMed:37731000
left-to-right
(2E)-butenoyl-CoA + L-lysyl-[protein] = N(6)-(2E)-butenoyl-L- lysyl-[protein] + CoA + H(+) - PubMed:25818647
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butanoyl-CoA + L-lysyl-[protein] = N(6)-butanoyl-L-lysyl- [protein] + CoA + H(+) - UniProtKB:B2RWS6
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propanoyl-CoA + L-lysyl-[protein] = N(6)-propanoyl-L-lysyl- [protein] + CoA + H(+) - PubMed:17267393
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2-hydroxyisobutanoyl-CoA + L-lysyl-[protein] = N(6)-(2- hydroxyisobutanoyl)-L-lysyl-[protein] + CoA + H(+) - PubMed:29775581
left-to-right
(S)-lactoyl-CoA + L-lysyl-[protein] = N(6)-[(S)-lactoyl]-L- lysyl-[protein] + CoA + H(+) - PubMed:31645732
left-to-right