Brand  (β version)

PDB ID: 5NPQ

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Ligands
Code Name Style Show Link
GOL Glycerol PoSSuM
94Q ~{S}-[2-[3-[[(2~{S})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 9-(3-but-3-ynyl-1,2-diazirin-3-yl)nonanethioate PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
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Structure summary

Code : 5NPQ   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Human N-myristoyltransferase 1 (MNT1) with Myristoyl-CoA analogue X10 bound
Release Data : 2018-05-30
Compound :
mol_id molecule chains synonym
1 Glycylpeptide N-tetradecanoyltransferase 1 A,B Myristoyl-CoA:protein N-myristoyltransferase 1,Type I N-myristoyltransferase,Peptide N-myristoyltransferase 1
ec: 2.3.1.97
fragment: human N-myristoyltransferase
mutation: This protein was produced with an N-terminal His-Tag followed by a 3C protease site. The His-tag was cleaved with 3C protease for crystallization leaving 3 residues that are followed by 109-496 amino acids of the NMT1
other_details: This protein was produced with an N-terminal His-Tag followed by a 3C protease site. The His-tag was cleaved with 3C protease for crystallization leaving 3 residues that are followed by 109-496 amino acids of the NMT1. The 3 residues preceding the NMT1 were not modeled as they were flexible and not observed in the electron density map. Residues 109-114, 182-188, 308-319 and 407-414 of the NMT1 chain A and residues 109-114 and 312-318 of the NMT1 chain B were not modeled because they were disordered and cannot be seen in the electron density map.
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli BL21(DE3)  (taxid:469008)
gene: NMT1, NMT
expression_system_variant: pRareS
expression_system_vector_type: plasmid
expression_system_plasmid: pET28
Authors : Shen, M., Perez-Dorado, I., Fedoryshchak, R., Tate, E.W.
Keywords : N-myristoylation, transferase, Myristol-CoA analogue, UV active, chemical probe
Exp. method : X-RAY DIFFRACTION ( 2.3720 Å )
Citation :

Human N-myristoyltransferase 1 (MNT1) with Myristoyl-CoA analogue X10 bound.

Shen, M.,Perez-Dorado, I.,Fedoryshchak, R.  et al.
To be published 

Reaction

Chain : A, B
UniProt : P30419 (NMT1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
N-terminal glycyl-[protein] + tetradecanoyl-CoA = CoA + H(+) + N-tetradecanoylglycyl-[protein] 2.3.1.97 PubMed:22865860, PubMed:25255805, PubMed:32103017, PubMed:32111831, PubMed:32686708, PubMed:34999170, PubMed:9353336, PubMed:9506952
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N-terminal glycyl-L-lysyl-[protein] + tetradecanoyl-CoA = CoA + H(+) + N-terminal glycyl-(N(6)-tetradecanoyl)-L-lysyl-[protein] - PubMed:32103017, PubMed:32111831
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