Brand  (β version)

PDB ID: 5MDJ

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Ligands
Code Name Style Show Link
CL Chloride ion PoSSuM
F3S Fe3-s4 cluster PoSSuM
F4S Fe4-s3 cluster PoSSuM
MG Magnesium ion PoSSuM
NFV Ni-Fe oxidized active center PoSSuM
SF4 Iron/sulfur cluster PoSSuM
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Structure summary

Code : 5MDJ   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in a its as-isolated high-pressurized form
Release Data : 2018-02-21
Compound :
mol_id molecule chains synonym
1 Uptake hydrogenase large subunit; HOXG L Hydrogenlyase,Membrane-bound hydrogenase large subunit
ec: 1.12.99.6
mol_id molecule chains synonym
2 Uptake hydrogenase small subunit; HOXK S Hydrogenlyase,Membrane-bound hydrogenase small subunit
ec: 1.12.99.6
Source :
mol_id organism_scientific expression_system
1 Ralstonia eutropha H16  (taxid:381666) Ralstonia eutropha H16  (taxid:381666)
atcc: 17699
gene: hoxG, PHG002
expression_system_strain: H16 DSM 428 STANIER 337
expression_system_atcc_number: 17699
expression_system_vector_type: PLASMID
mol_id organism_scientific expression_system
2 Ralstonia eutropha H16  (taxid:381666) Ralstonia eutropha H16  (taxid:381666)
atcc: 17699
gene: hoxK, PHG001
expression_system_strain: H16 DSM 428 STANIER 337
expression_system_atcc_number: 17699
expression_system_vector_type: PLASMID
Authors : Schmidt, A., Kalms, J., Scheerer, P.
Keywords : [NIFE] HYDROGENASE, KNALLGASBACTERIA, PROTEOBACTERIA, AEROBIC HYDROGEN BACTERIA, HIGH PRESSURE PUMPING, DEHYDROGENASE, OXIDOREDUCTASE, HYDROGEN CATALYSIS, METALLOENZYME, DIHYDROGEN, BIMETALLIC, METALLOPROTEIN CATALYTIC CENTER, NI-FE ACTIVE SITE, T-CLUSTER, FE-S CLUSTER, [4Fe-3S] CLUSTER, [3Fe-4S] CLUSTER, [4Fe-4S] CLUSTER, AS-ISOLATED STATE, OXIDIZED STATE, OXYGEN TOLERANT HYDROGENASE, MEMBRANE BOUND, MEMBRANE, HYDROPHOBIC TUNNEL, HIGH PRESSURE CRYO COOLING, GAS TRANSPORT
Exp. method : X-RAY DIFFRACTION ( 1.48 Å )
Citation :

Tracking the route of molecular oxygen in O2-tolerant membrane-bound [NiFe] hydrogenase.

Kalms, J.,Schmidt, A.,Frielingsdorf, S.  et al.
(2018)  Proc. Natl. Acad. Sci. U.S.A.  115 : E2229 - E2237

PubMed: 29463722
DOI: 10.1073/pnas.1712267115

Reaction

Chain : L
UniProt : P31891 (MBHL_CUPNH)
Reaction: EC: Evidence:
Physiological Direction:
A + H2 = AH2 1.12.99.6 -
-
Chain : S
UniProt : P31892 (MBHS_CUPNH)
Reaction: EC: Evidence:
Physiological Direction:
A + H2 = AH2 1.12.99.6 -
-