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Ligands
Code Name Style Show Link
CBY Cob(II)inamide
CYN Cyanide ion
GOL Glycerol
Non-standard Residues
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Glycosylation
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Modification
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Code : 5M34   PDBj   RCSB PDB   PDBe
Header : TRANSPORT PROTEIN
Title : Structure of cobinamide-bound BtuF mutant W66Y, the periplasmic vitamin B12 binding protein in E.coli
Release Data : 2017-03-01
Compound :
mol_id molecule chains
1 Vitamin B12-binding protein A,B
other_details: OmpA-NcoI-BtuF-BamHI-3C-BamHI-His6
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:83333) Escherichia coli  (taxid:562)
strain: K12
gene: btuF, yadT, b0158, JW0154
Authors : Mireku, S.A., Ruetz, M., Zhou, T., Korkhov, V.M., Kraeutler, B., Locher, K.P.
Keywords : BtuF, Cobinamide, periplasmic binding protein, ABC transporter, Transport protein
Exp. method : X-RAY DIFFRACTION ( 1.60 Å )
Citation :

Conformational Change of a Tryptophan Residue in BtuF Facilitates Binding and Transport of Cobinamide by the Vitamin B12 Transporter BtuCD-F.

Mireku, S.A.,Ruetz, M.,Zhou, T.  et al.
(2017)  Sci Rep  7 : 41575 - 41575

PubMed: 28128319
DOI: 10.1038/srep41575

Chain : A, B
UniProt : P37028 (BTUF_ECOLI)
Reaction : -