Brand  (β version)

PDB ID: 5LHQ

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Ligands
Code Name Style Show Link
0GJ L-alpha-glutamyl-N-{(1s)-4-{[amino(iminio)methyl]amino}-1-[(1s)-2-chloro-1-hydroxyethyl]butyl}glycinamide PoSSuM
EDO 1,2-ethanediol PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Structure summary

Code : 5LHQ   PDBj   RCSB PDB   PDBe
Header : Hydrolase/Antibody
Title : The EGR-cmk active site inhibited catalytic domain of murine urokinase-type plasminogen activator in complex with the allosteric inhibitory nanobody Nb7
Release Data : 2017-06-28
Compound :
mol_id molecule chains synonym
1 Urokinase-type plasminogen activator A uPA
ec: 3.4.21.73
mutation: C122A
mol_id molecule chains
2 Camelid-Derived Antibody Fragment Nb7 B
Source :
mol_id organism_scientific organism_common expression_system
1 Mus musculus  (taxid:10090) House Mouse Escherichia coli  (taxid:562)
gene: Plau
expression_system_vector_type: Plasmid
expression_system_vector: pT7
mol_id organism_scientific organism_common expression_system
2 Vicugna pacos  (taxid:30538) Alpaca Escherichia coli  (taxid:562)
Authors : Kromann-Hansen, T., Lange, E.L., Sorensen, H.P., Ghassabeh, G.H., Huang, M., Jensen, J.K., Muyldermans, S., Declerck, P.J., Andreasen, P.A.
Keywords : Trypsin-like serine proteases, Nanobody, Inhibitor, Hydrolase-Antibody complex
Exp. method : X-RAY DIFFRACTION ( 2.600 Å )
Citation :

Discovery of a novel conformational equilibrium in urokinase-type plasminogen activator.

Kromann-Hansen, T.,Louise Lange, E.,Peter Srensen, H.  et al.
(2017)  Sci Rep  7 : 3385 - 3385

PubMed: 28611361
DOI: 10.1038/s41598-017-03457-7

Reaction

Chain : A
UniProt : P06869 (UROK_MOUSE)
Reaction: EC: Evidence:
Physiological Direction:
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin. 3.4.21.73 -
-