Brand  (β version)

PDB ID: 5LBX

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Ligands
Code Name Style Show Link
CL Chloride ion PoSSuM
COO Crotonyl coenzyme a PoSSuM
NAP Nadp nicotinamide-adenine-dinucleotide phosphate PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 5LBX   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Structure of the T175V Etr1p mutant in the trigonal form P312 in complex with NADP and crotonyl-CoA
Release Data : 2017-05-10
Compound :
mol_id molecule chains synonym
1 Enoyl-[acyl-carrier-protein] reductase [NADPH, B-specific] 1, mitochondrial A,B Trans-2-enoyl-CoA reductase 1
ec: 1.3.1.10,1.3.1.38
mutation: T175V
other_details: The 20 first residues are not visible in the crystal structure. The sequence corresponds to the fragment 23-386 from the original gene sequence.
Source :
mol_id organism_scientific organism_common expression_system
1 Candida tropicalis  (taxid:5482) Yeast Escherichia coli 'BL21-Gold(DE3)pLysS AG'  (taxid:866768)
gene: ETR1
expression_system_vector_type: plasmid
expression_system_plasmid: pTE264
Authors : Wagner, T., Rosenthal, R.G., Voegeli, B., Shima, S., Erb, T.J.
Keywords : Negative catalysis, enzyme, NADP, mitochondria, mutant, crotonyl-coa, reductase, oxidoreductase
Exp. method : X-RAY DIFFRACTION ( 2.500 Å )
Citation :

A conserved threonine prevents self-intoxication of enoyl-thioester reductases.

Rosenthal, R.G.,Vogeli, B.,Wagner, T.  et al.
(2017)  Nat. Chem. Biol.  13 : 745 - 749

PubMed: 28504678
DOI: 10.1038/nchembio.2375

Reaction

Chain : A, B
UniProt : Q8WZM3 (ETR1_CANTR)
Reaction: EC: Evidence:
Physiological Direction:
a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + H(+) + NADPH 1.3.1.104 PubMed:12890667
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(2E,4E)-hexadienoyl-CoA + H(+) + NADPH = (4E)-hexenoyl-CoA + NADP(+) - PubMed:11509667
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(2E)-hexenoyl-CoA + H(+) + NADPH = hexanoyl-CoA + NADP(+) - PubMed:11509667
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