Brand  (β version)

PDB ID: 5KTS

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Ligands
Code Name Style Show Link
CIZ (~{z})-2-methylbut-2-enedioic acid PoSSuM
CL Chloride ion PoSSuM
NH4 Ammonium ion PoSSuM
SF4 Iron/sulfur cluster PoSSuM
Non-standard Residues
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Structure summary

Code : 5KTS   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of Pyrococcus horikoshii quinolinate synthase (NadA) with bound citraconate and Fe4S4 cluster
Release Data : 2016-07-27
Compound :
mol_id molecule chains
1 Quinolinate synthase A A
ec: 2.5.1.72
Source :
mol_id organism_scientific expression_system
1 Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)  (taxid:70601) Escherichia coli BL21(DE3)  (taxid:469008)
strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3
gene: nadA, PH0013
expression_system_vector_type: Plasmid
expression_system_plasmid: pDESTF1
Authors : Fenwick, M.K., Ealick, S.E.
Keywords : Dehydratase, iron-sulfur cluster, substrate analog complex, biosynthetic enzyme, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 1.34 Å )
Citation :

Crystal Structures of the Iron-Sulfur Cluster-Dependent Quinolinate Synthase in Complex with Dihydroxyacetone Phosphate, Iminoaspartate Analogues, and Quinolinate.

Fenwick, M.K.,Ealick, S.E.
(2016)  Biochemistry  55 : 4135 - 4139

PubMed: 27404889
DOI: 10.1021/acs.biochem.6b00626

Reaction

Chain : A
UniProt : O57767 (NADA_PYRHO)
Reaction: EC: Evidence:
Physiological Direction:
iminosuccinate + dihydroxyacetone phosphate = quinolinate + phosphate + 2 H2O + H(+) 2.5.1.72 HAMAP-Rule:MF_00568, PubMed:15937336, PubMed:27224840
left-to-right HAMAP-Rule:MF_00568, PubMed:15937336
Cofactor: Evidence: Note:
[4Fe-4S] cluster ECO:0000255 | HAMAP-Rule:MF_00568
ECO:0000269 | PubMed:27224840
ECO:0000269 | PubMed:27404889
ECO:0000269 | PubMed:31390192
Binds 1 [4Fe-4S] cluster per subunit.