Brand  (β version)

PDB ID: 5KKB

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Ligands
Code Name Style Show Link
LA Lanthanum (III) ion PoSSuM
1PS 3-pyridinium-1-ylpropane-1-sulfonate PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
NAG 2-acetamido-2-deoxy-beta-D-glucopyranose
BMA Beta-D-mannopyranose
MAN Alpha-D-mannopyranose
Modification
Code Name Show

Structure summary

Code : 5KKB   PDBj   RCSB PDB   PDBe
Header : SUGAR BINDING PROTEIN
Title : Structure of mouse Golgi alpha-1,2-mannosidase IA and Man9GlcNAc2-PA complex
Release Data : 2016-12-07
Compound :
mol_id molecule chains synonym
1 Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA A,B Man(9)-alpha-mannosidase,Man9-mannosidase,Mannosidase alpha class 1A member 1,Processing alpha-1,2-mannosidase IA,Alpha-1
ec: 3.2.1.113
Source :
mol_id organism_scientific organism_common expression_system
1 Mus musculus  (taxid:10090) Mouse Komagataella pastoris  (taxid:4922)
gene: Man1a1, Man1a
Authors : Xiang, Y., Moremen, K.W.
Keywords : Alpha/alpha barrel, Golgi apparatus, deglycosylation, glycan substrate analog, SUGAR BINDING PROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.774 Å )
Citation :

Substrate recognition and catalysis by GH47 alpha-mannosidases involved in Asn-linked glycan maturation in the mammalian secretory pathway.

Xiang, Y.,Karaveg, K.,Moremen, K.W.
(2016)  Proc. Natl. Acad. Sci. U.S.A.  113 : E7890 - E7899

PubMed: 27856750
DOI: 10.1073/pnas.1611213113

Reaction

Chain : A, B
UniProt : P45700 (MA1A1_MOUSE)
Reaction: EC: Evidence:
Physiological Direction:
N(4)-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man- (1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)- alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D- GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1->3)- [alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D- Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl- [protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose 3.2.1.113 UniProtKB:P32906
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N(4)-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man- (1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]- alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D- GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man- (1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)- beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose 3.2.1.113 UniProtKB:P32906
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Cofactor: Evidence: Note:
Ca(2+) ECO:0000269 | PubMed:15102839
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