Brand  (β version)

PDB ID: 5KIJ

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Ligands
Code Name Style Show Link
1PS 3-pyridinium-1-ylpropane-1-sulfonate PoSSuM
BU1 1,4-butanediol PoSSuM
LA Lanthanum (III) ion PoSSuM
SO4 Sulfate ion PoSSuM
BMA Beta-D-mannopyranose PoSSuM
MAN Alpha-D-mannopyranose PoSSuM
NAG 2-acetamido-2-deoxy-beta-D-glucopyranose PoSSuM
: Polysaccharide
Non-standard Residues
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Glycosylation
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Structure summary

Code : 5KIJ   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase and Man9GlcNAc2-PA complex
Release Data : 2017-05-10
Compound :
mol_id molecule chains synonym
1 Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase A ER alpha-1,2-mannosidase,ER mannosidase 1,ERMan1,Man9GlcNAc2-specific-processing alpha-mannosidase,Mannosidase alpha class 1B member 1
ec: 3.2.1.113
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Komagataella pastoris  (taxid:4922)
gene: MAN1B1, UNQ747/PRO1477
Authors : Karaveg, K., Xiang, Y., Moremen, K.W.
Keywords : alpha/alpha-barrel, cation replacement, protein-glycan interaction, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.649 Å )
Citation :

Substrate recognition and catalysis by GH47 alpha-mannosidases involved in Asn-linked glycan maturation in the mammalian secretory pathway.

Xiang, Y.,Karaveg, K.,Moremen, K.W.
(2016)  Proc. Natl. Acad. Sci. U.S.A.  113 : E7890 - E7899

PubMed: 27856750
DOI: 10.1073/pnas.1611213113

Reaction

Chain : A
UniProt : Q9UKM7 (MA1B1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
4 H2O + N(4)-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D- Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man- (1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)- beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 beta-D-mannose + N(4)-(alpha-D-Man- (1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]- beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl- [protein] (N-glucan mannose isomer 5A1,2) 3.2.1.113 PubMed:10409699, PubMed:12090241, PubMed:15713668
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3 H2O + N(4)-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D- Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man- (1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)- beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 beta-D-mannose + N(4)-(alpha-D-Man-(1->3)-[alpha-D- Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man- (1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) 3.2.1.113 PubMed:10409699, PubMed:12090241, PubMed:15713668
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