Brand  (β version)

PDB ID: 5K3S

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Ligands
Code Name Style Show Link
6QL 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid PoSSuM
FAD Flavin-adenine dinucleotide PoSSuM
MG Magnesium ion PoSSuM
TP9 (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate PoSSuM
Non-standard Residues
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CSD 3-sulfinoalanine
Glycosylation
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Structure summary

Code : 5K3S   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of Arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium
Release Data : 2017-02-08
Compound :
mol_id molecule chains synonym
1 Acetolactate synthase, chloroplastic A AtALS,Acetohydroxy-acid synthase,Protein CHLORSULFURON RESISTANT 1
ec: 2.2.1.6
fragment: UNP residues 86-667
Source :
mol_id organism_scientific organism_common expression_system
1 Arabidopsis thaliana  (taxid:3702) Mouse-ear cress Escherichia coli BL21(DE3)  (taxid:469008)
gene: ALS, AHAS, CSR1, TZP5, At3g48560, T8P19.70
expression_system_strain: BL21(DE3)
expression_system_vector_type: Plasmid
expression_system_plasmid: PET30A(+)
Authors : Garcia, M.D., Lonhienne, T., Guddat, L.W.
Keywords : AHAS, acetohydroxyacid synthase, acetolactate synthase, herbicide, bispyribac-sodium, thiamin diphosphate, FAD, pyrimidinyl-benzoate, transferase
Exp. method : X-RAY DIFFRACTION ( 2.873 Å )
Citation :

Comprehensive understanding of acetohydroxyacid synthase inhibition by different herbicide families.

Garcia, M.D.,Nouwens, A.,Lonhienne, T.G.  et al.
(2017)  Proc. Natl. Acad. Sci. U.S.A.  114 : E1091 - E1100

PubMed: 28137884
DOI: 10.1073/pnas.1616142114

Reaction

Chain : A
UniProt : P17597 (ILVB_ARATH)
Reaction: EC: Evidence:
Physiological Direction:
2 pyruvate + H(+) = (2S)-2-acetolactate + CO2 2.2.1.6 -
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000269 | PubMed:32640464
Binds 1 Mg(2+) ion per subunit.
FAD ECO:0000269 | PubMed:32640464
Binds 1 Mg(2+) ion per subunit.
thiamine diphosphate ECO:0000269 | PubMed:32640464
Binds 1 thiamine pyrophosphate per subunit.