Brand  (β version)

PDB ID: 5IK6

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Ligands
Code Name Style Show Link
CRE Germacrene a PoSSuM
DPO Diphosphate PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 5IK6   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : Tobacco 5-epi-aristolochene synthase with germacrene A and PPi
Release Data : 2016-10-05
Compound :
mol_id molecule chains synonym
1 5-epi-aristolochene synthase A EAS
ec: 4.2.3.61
Source :
mol_id organism_scientific organism_common expression_system
1 Nicotiana tabacum  (taxid:4097) Common tobacco Escherichia coli  (taxid:469008)
gene: EAS3, EAS4
expression_system_strain: BL21 (DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pH9GW
Authors : Koo, H.J., Xu, Y., Louie, G.V., Bowman, M., Noel, J.P.
Keywords : terpene synthase, TEAS, germacrene A, diphosphate, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.300 Å )
Citation :

Biosynthetic potential of sesquiterpene synthases: product profiles of Egyptian Henbane premnaspirodiene synthase and related mutants.

Koo, H.J.,Vickery, C.R.,Xu, Y.  et al.
(2016)  J.Antibiot.  69 : 524 - 533

PubMed: 27328867
DOI: 10.1038/ja.2016.68

Reaction

Chain : A
UniProt : Q40577 (5EAS_TOBAC)
Reaction: EC: Evidence:
Physiological Direction:
(2E,6E)-farnesyl diphosphate = (+)-5-epi-aristolochene + diphosphate 4.2.3.61 PubMed:16375847, PubMed:20175559, PubMed:25897591, PubMed:26378620, Ref.3
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(2Z,6E)-farnesyl diphosphate = (+)-2-epi-prezizaene + diphosphate 4.2.3.226 PubMed:20175559, PubMed:20201526
left-to-right
(2Z,6E)-farnesyl diphosphate = (-)-alpha-cedrene + diphosphate 4.2.3.227 PubMed:20175559, PubMed:20201526
left-to-right
(2Z,6E)-farnesyl diphosphate = (-)-beta-curcumene + diphosphate - PubMed:20175559, PubMed:20201526
left-to-right
Cofactor: Evidence: Note:
Mg(2+) ECO:0000269 | PubMed:9295271
Binds 3 Mg(2+) ions per subunit.