Brand  (β version)

PDB ID: 5G6D

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
CL Chloride ion PoSSuM
GOL Glycerol PoSSuM
H4B 5,6,7,8-tetrahydrobiopterin PoSSuM
HEM Protoporphyrin ix containing Fe PoSSuM
JHT 7-[[[3-(dimethylamino)phenyl]methylamino]methyl]quinolin-2-amine PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 5G6D   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Structure of Bacillus subtilis Nitric Oxide Synthase in complex with 7-(((3-(Dimethylamino)benzyl)amino)methyl)quinolin-2-amine
Release Data : 2016-09-21
Compound :
mol_id molecule chains synonym
1 NITRIC OXIDE SYNTHASE OXYGENASE A NOSOXY-LIKE PROTEIN, NITRIC OXIDE SYNTHASE
ec: 1.14.13.165
mutation: YES
Source :
mol_id organism_scientific expression_system
1 BACILLUS SUBTILIS  (taxid:1423) ESCHERICHIA COLI  (taxid:469008)
strain: 168
atcc: 23857
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_vector: PET28A
Authors : Holden, J.K., Poulos, T.L.
Keywords : NITRIC OXIDE SYNTHASE, OXIDOREDUCTASE, INHIBITOR
Exp. method : X-RAY DIFFRACTION ( 1.821 Å )
Citation :

Targeting Bacterial Nitric Oxide Synthase with Aminoquinoline-Based Inhibitors.

Holden, J.K.,Lewis, M.C.,Cinelli, M.A.  et al.
(2016)  Biochemistry  55 : 5587 - 5594

PubMed: 27607918
DOI: 10.1021/acs.biochem.6b00786

Reaction

Chain : A
UniProt : O34453 (NOSO_BACSU)
Reaction: EC: Evidence:
Physiological Direction:
3 reduced [flavodoxin] + 2 L-arginine + 4 O2 = 3 oxidized [flavodoxin] + 2 L-citrulline + 2 nitric oxide + 4 H2O + 5 H(+) 1.14.14.47 PubMed:11856757
-
Cofactor: Evidence: Note:
heme ECO:0000269 | PubMed:11856757
-
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin ECO:0000269 | PubMed:11856757
Tetrahydrobiopterin (H4B). Can also use tetrahydrofolate (THF) in place of tetrahydrobiopterin (H4B) but binds THF with much lower affinity than H4B.